离子液体/表面活性剂协同增敏荧光法测定米力农  

Determination of milrinone content by RTILs/surfactant synergistic sensitized spectrofluorimetry

作　　者：龚爱琴[1] 王元有[1] 姜业朝[1] GONG Ai-qin;WANG Yuan-you;JIANG Ye-chao(Yangzhou Polytechnic Institute, Yangzhou 225127)

机构地区：[1]扬州工业职业技术学院,扬州225127

出　　处：《分析试验室》2018年第6期651-655,共5页Chinese Journal of Analysis Laboratory

基　　金：江苏省青蓝工程(苏教师(2016)15号);扬州工业职业技术学院校级科研课题(2016xjzk008)项目资助

摘　　要：基于米力农自身荧光在十六烷基三甲基溴化铵(CTAB)和室温离子液体(RTILs)溴化十二烷基三甲基咪唑混合体系中得到显著增强,提出了测定药物及生物体液中米力农含量的荧光新方法。在优化条件下,线性方程为F=47.32+1378.65ρ(ρ:μg/mL,r=0.998),线性范围为0.02~1.5μg/mL,检出限为6.4 ng/mL。实验中对荧光增敏机理进行了探讨。将方法用于米力农注射液和人血清、尿液中米力农含量的测定,回收率在97.5%~107.4%,米力农注射液测定结果与法定方法一致。Based on the fluorescence of milrinone could be significantly enhanced in the medium of hexadecyl trimethyl ammonium bromide（CTAB）/room temperature ionic liquids（RTILs）（[C12 mim]Br）,a simple spectrofluorimetric method to determine milrinone in milrinone injection and body fluids was developed.The various factors of influencing determination were discussed.Under the optimal conditions,a linear calibration curve（F = 47.32 ＋ 1378.65ρ,ρ： μg/mL） was obtained in the range of 0.02 ～ 1.5 μg/mL and the detection limit was 6.4 ng/mL（r = 0.998）.The preliminary sensitized mechanism was discussed.The studied method was successfully used to determine milrinone in milrinone injection and human serum/urine samples with the recovery of 97.5% ～ 107.4%.The obtained results of milrinone lactate injection were in good agreement with the results of official method.

关 键 词：米力农 室温离子液体 表面活性剂 协同增敏 荧光法 

分 类 号：TQ460.7[化学工程—制药化工]