纳米金-重组高密度脂蛋白CT靶向对比剂的动物实验研究  

Preliminary study of CT targeted contrast agent of gold nanoparticles-recombinant high-density lipoprotein in animal model

作　　者：李江[1] 辛越[1] 秦健[1] 朱建忠[1] LI Jiang;XIN Yue;QIN Jian;ZHU Jianzhong(Department of Radiology,the Affiliated Hospital of Taishan Medical University,Tai ＇an 271000,China)

机构地区：[1]泰山医学院附属医院医学影像科,山东泰安271000

出　　处：《实用放射学杂志》2018年第6期951-954,共4页Journal of Practical Radiology

基　　金：山东省自然科学基金项目（ZR2014HL085）.

摘　　要：目的 探讨纳米金-重组高密度脂蛋白（Au-rHDL）CT靶向对比剂对活体动脉粥样硬化（AS）斑块内巨噬细胞的分子影像学成像及病理对照分析。方法 制备Au-rHDL靶向对比剂及AS动物模型；活体动物模型斑块内巨噬细胞靶向成像与病理切面匹配，分析巨噬细胞分布与斑块负荷的相关性及一致性。结果 ①Au-rHDL靶向对比剂具有生物学活性，且能够特异地与斑块内巨噬细胞相结合；②巨噬细胞的定位以及斑块负荷的结果无统计学差异（P=0.863、0.726），并且2种方法所得结果有较高的相关性（r=0.897、0.736）和一致性（ICC=0.885、0.763）。结论 基于Au-rHDL靶向CT对比剂的分子影像技术，能够实现影像学对粥样硬化斑块内巨噬细胞的识别及定位分析，为AS斑块预警及易损性的风险预测提供理论基础。Objective To investigate CT targeted contrast agent of gold nanoparticles-recombinant high-density lipoprotein （Au-rHDL） in analyzing the relationship between molecular imaging and pathology for macrophages in atherosclerotic plaques in living animals. Methods Au rHDL targeted contrast agents and atherosclerotic animal models were obtained. Targeted images for macrophages in living animal models were matched with the corresponding pathological sections, and the correlation and consistency between macrophages distribution and plaque burden were analyzed. Results ① Au-rHDL targeted contrast agent had biological activity, which could specifically combine with macrophages in atheroselerotic plaques;②There was no statistical difference between the location of maerophages and the results of plaque burden （P =0.863, 0.726）, and the results of two methods had high correlation （r=0.897,0.736） and consistency （ ICC=0.885, 0.763 ）. Conclusion The molecular imaging technology basing on CT targeted contrast agent of Au-rHDL can be used for analyzing the identification and localization of macrophages in atherosclerotic plaque, and providing a theoretical basis for early warning and vulnerability risk prediction of atherosclerotic plaques.

关 键 词：分子影像学 纳米金 靶向对比剂 粥样硬化斑块 巨噬细胞 

分 类 号：R814.42[医药卫生—影像医学与核医学] R543.5[医药卫生—放射医学]