亚硝酸盐氮对团头鲂急性毒性及高铁血红蛋白的影响  

Acute Toxicity of Nitrite on Megalobrama amblycephala and Effect on Serum Methemoglobin

作　　者：滕涛[1,2] 习丙文[1,2] 费茜旎 谢骏[1,2] 徐跑[1,2] TENG Tao;XI Bing-wen;FEI Qian-ni;XIE Jun;XU Pao(Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, P. R. China;Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 ,P. R. China)

机构地区：[1]南京农业大学无锡渔业学院,江苏无锡214081 [2]中国水产科学研究院淡水渔业研究中心,农业部淡水渔业和种质资源利用重点实验室,江苏无锡214081

出　　处：《水生态学杂志》2018年第3期99-106,共8页Journal of Hydroecology

基　　金：现代农业产业技术体系建设专项资金(CARS-46-10)

摘　　要：为进一步了解不同浓度亚硝酸盐氮（NO-2-N）对团头鲂（Megalobrama amblycephala）急性毒性及高铁血红蛋白的影响,通过设置不同浓度的Na NO2胁迫浓度,开展急性毒性试验,检测不同时间下团头鲂血液生化指标的变化情况。结果表明,NO-2-N对体重（20.0±1.0）g团头鲂幼鱼24、48、72、96 h LC50及安全浓度（CS）分别为42.2 mg/L、35.9 mg/L、32.5 mg/L、30.1 mg/L和3.0 mg/L。实验鱼在12.0 mg/L、9.0 mg/L、6.0 mg/L、3.0 mg/L的浓度胁迫下,其血液生化指标均发生明显的变化;实验组12.0 mg/L、9.0 mg/L在胁迫24 h后白细胞数量、红细胞数量、血细胞比容与对照组相比均差异性显著（P〈0.05）。在胁迫24 h后,血红蛋白含量与对照组相比均差异性不显著（P〉0.05）,但有上升趋势。随着胁迫浓度升高,时间延长,血红蛋白含量降低,高铁血红蛋白比率升高,血红蛋白大量转化为高铁血红蛋白,并且72 h后胁迫浓度组6.0 mg/L、9.0 mg/L、12.0 mg/L均与对照组差异性显著（P〈0.05）。本实验为深入了解NO-2-N对团头鲂的致毒机理提供基础数据,为养殖环境控制和疾病防控提供依据。Nitrite is toxic to aquatic organisms. High protein inputs characteristic of intensive aquaculture lead to high nitrite concentrations in ponds, especially at elevated water temperatures, and pose a serious threat for the aquaculture industry. This study examined the acute toxicity of nitrite on Megalobrarna amblycephala and its impact on serum methemoglobin. The juvenile fish （20.0 ± 1.0 g） were acclimatized in dechlorinated tap water at 26 ± 0.5℃, pH = 7.5 and DO ≥5 mg/L. Nitrite concentrations in the exposure tanks were adjusted with sodium nitrite （ NaNO2）. Exposure levels were set at 12.0 mg/L, 9.0 mg/L, 6.0 mg/L, 3.0 mg/L and 0.0 mg/L （control）. The median lethal concentration （LC50） to M. amhlyeephala at 24, 48, 72, 96 h were, respectively, 42.2 mg/L, 35.9 mg/L, 32.5 mg/L, 30.1 mg/L. The concentration considered safe is 3.0 mg/L. Changes in complete blood count （CBC） were dose dependent. Significant changes occurred within 24 h at nitrite exposure levels of 12.0 mg/L and 9.0 mg/L; white blood cell （WBC） and red blood cell （RBC） counts and hematocrit were all significantly different from the control （P 〈 0.05 ）. Hemoglobin （Hb） also increased, but not significantly. After exposure to 12.0 mg/L of nitrite for 24 h, the ratio of methemoglobin to hemoglobin （MetHb/Hb） increased significantly （ P 〈 0. 05 ） and, after 72 h, the increase was also significant at exposure levels of 6.0 mg/L and 9.0 mg/L. These results will play an important role in developing standards necessary to prevent the occurrence of toxic levels of nitrite in aquacuhural waters and prevent environmentally related disease.

关 键 词：亚硝酸盐氮 团头鲂 急性毒性 高铁血红蛋白 血液生化指标 

分 类 号：Q592[生物学—生物化学]