基于热带玉米种质的高效诱导自交系筛选  

Screening of Inbred Lines with Higher Induced Rate by Using Tropical Maize Germplasm to be Acceptor Parent

作　　者：吴迅 杨克相 郭向阳[1] 王安贵[1] 刘鹏飞 李秀诗 祝云芳[1] 陈泽辉[1] WU Xun;YANG Ke-xiang;GUO Xiang-yang;WANG An-gui;LIU Peng-fei;LI Xiu-shi;ZHU Yun-fang;CHEN Ze-hui(Institute of Upland Food Crops, Guizhou Academy of Agricultural Science, Guiyang 550006;Oil Crops Research Institute, Guizhou Academy of Agricultural Science, Guiyang 550006, China)

机构地区：[1]贵州省农业科学院旱粮研究所,贵阳550006 [2]贵州省农业科学院油料研究所,贵阳550006

出　　处：《玉米科学》2018年第3期17-21,共5页Journal of Maize Sciences

基　　金：黔科合基础[2017]1413;黔科合支撑[2018]2296号;黔科合支撑[2016]2605号;黔科合服企[2014]4002;贵州省农业攻关项目[2016]2549;贵州省农科院扶贫专项

摘　　要：分别以热带玉米群体苏湾1号、苏兰3号和QR273/LCH645的F2后代为受体亲本,4份具有不同来源的诱导系作父本,组配规模为20个果穗/组合的杂交后代。根据子粒的胚和胚乳显色差异鉴定出单倍体子粒,评估不同诱导系和母本群体的诱导率。结果表明,苏湾1号呈现出较其改良材料低的诱导率,随着温带血缘的增加,诱导率逐渐增加。针对相同受体材料,不同诱导系的诱导率之间存在显著差异,其中,诱导系1619表现出最高的诱导率（6.28±0.20-7.94±0.85）,诱导系1613表现出最低的诱导率（1.93±0.19-3.68±0.05）,诱导系1619在诱导热带种质产生单倍体方面具有较高的效率。Fifty crosses for each pair-parent were constructed, by using tropical maize germplasm of Suwan1,Sulan3, and the F2 progeny of QR273 crossed with LCH645, four induced lines from different resources to be themale parent. Each haploid kernel was identified based on the color performance of the kernel, with purple endo-sperm and white embryo, after pollination. Based on this, the induced rate for each inducer was calculated. The re-sult showed that, lower induced rate was found for Suwan1, and the induced rate was increasing with the increasedintrogression of temperate gene resources. For identical acceptor parent, remarkable difference of induced rate wasfound between different induced lines. Where in 1619 showed the highest value, with the induced rate varied from6.28±0.20 to 7.94±0.85, inducer line of 1613 showed the lowest value, with induced rate varied from 1.93±0.19 to3.68±0.05, which clarified that 1619 is better for the inducing of diploid lines for tropical germplasm.

关 键 词：玉米 苏湾种质 单倍体技术 诱导率 遗传改良 

分 类 号：S513.035.2[农业科学—作物学]