瘢痕灸对原发性肝癌大鼠β-catenin及sFRP1表达的影响  

Effects of Scarring Moxibustion on Expressions of β-catenin and sFRP1 with Hepatic Carcinoma Rats

作　　者：王琪格 陈金 丁思元 李迪[2] 赵万爽 李梦迪[2] 关圣涛[2] 王颖[2] WANG Qige;CHEN Jin;DING Siyuan;LI Di;ZHAO Wanshuang;LI Mengdi;GUAN Shengtao;WANG Ying(The First Clinical Medical College, Liaoning University of TCM, Shenyang 110847, Liaoning, China;Acupuncture and Moxibustion and Tuina College, Liaoning University of TCM, Shenyang 110847, Liaoning, China)

机构地区：[1]辽宁中医药大学第一临床学院,辽宁沈阳110847 [2]辽宁中医药大学针灸推拿学院,辽宁沈阳110847

出　　处：《中华中医药学刊》2018年第6期1305-1308,I0005,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2012CB518500);辽宁省科学技术基金项目(2014020047)

摘　　要：目的：探讨瘢痕灸对肝癌大鼠肝脏组织中Wnt/β-catenin信号通路中的β-连环蛋白（β-catenin）及分泌型卷曲相关蛋白（sFRP1）表达的影响。方法：将60只SPF级Wistar雄性大鼠按随机数字表分为空白组、模型组、阿霉素组、足三里组和肝俞组,每组12只。分别给予相应干预后,采用Western Blot检测β-catenin、sFRP1的表达。结果：与空白组相比,模型组、阿霉素组、足三里组和肝俞组的β-catenin的表达量明显升高（P〈0.05）,sFRP1的表达量下降（P〈0.05）;与模型组比较,阿霉素组、足三里组和肝俞组的β-catenin的表达幅度降低（P〈0.05）,sFRP1的表达量升高（P〈0.05）;与阿霉素组比较,足三里组和肝俞组的β-catenin的表达量显著降低（P〈0.05）,sFRP1的表达幅度升高（P〈0.05）;与足三里组比较,肝俞组的β-catenin的表达量显著降低（P〈0.05）,sFRP1的表达量明显升高（P〈0.05）。结论：瘢痕灸对原发性肝癌大鼠有一定治疗效果,且瘢痕灸肝俞穴疗效较足三里穴更为显著。Objective：To investigate the effects of scarring moxibustion on the expression of β-catenin and sFRP1 in Wnt/β-catenin signaling pathway with hepatic carcinoma rats. Methods：Sixty SPF Wistar male rats were randomly divided into blank group,model group,Adriamycin group,Zusanli（ ST36） group and Ganshu（ BL18） group,12 rats in each group. The expressions of β-catenin and sFRP1 were detected by Western blot after corresponding intervention.Results：Compared with the blank group,the expressions of β-catenin in model group,Adriamycin group,Zusanli（ST36） group and Ganshu（BL18） group were significantly increased（P〈0. 05） and the expression of sFRP1 was significantly decreased（ P〈0. 05）. Compared with the model group,the expression of β-catenin was significantly decreased in the Adriamycin group,Zusanli（ ST36） group and Ganshu（ BL18） group（ P〈0. 05）,and the expression of sFRP1 was significantly increased（ P 〈0. 05）. Compared with Adriamycin group,the expressions of β-catenin in Zusanli（ ST36） and Ganshu（ BL18） were significantly decreased（ P 〈0. 05）,and the expression of sFRP1 was significantly increased（ P〈 0. 05）. Compared with Zusanli（ ST36） group,the expression of β-catenin in Ganshu（ BL18） was significantly decreased（ P〈 0. 05） and the expression of sFRP1 was significantly increased（ P 〈0. 05）. Conclusions：Scarring moxibustion has a certain effect on hepatic carcinoma rats and scarring moxibustion on Ganshu（ BL18） is more effective than that on Zusanli（ ST36）.

关 键 词：瘢痕灸 肝俞 足三里 原发性肝癌 Β-CATENIN sFRPl 

分 类 号：R285.5[医药卫生—中药学]