cAMP-PKA信号通路参与电针调控兔退变椎间盘AQP1、AQP3表达的机制研究  

c AMP-PKA Signal Pathway Involved in Electroacupuncture Regulating Expressions of AQP1 and AQP3 of Rabbits’ Disc Degeneration

作　　者：廖明轩 邹璟[2] 黄国付[2,3] LIAO Mingxuan;ZOU Jing;HUANG Guofu(Acupuncture - Moxibustion and Orthopaedics College, Hubei University of TCM, Wuhan 430065, Hubei, China;Wuhan First Hospital, Wuhan 430022, Hubei, China;Wuchang Hospital, Wuhan 430063, Hubei, China)

机构地区：[1]湖北中医药大学针灸骨伤学院,湖北武汉430065 [2]武汉市第一医院,湖北武汉430022 [3]武汉市武昌医院,湖北武汉430063

出　　处：《中华中医药学刊》2018年第6期1320-1323,I0012,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81574072);湖北省自然科学基金项目(2014CFA088)

摘　　要：目的：探讨cAMP-PKA信号通路参与电针调控兔退变椎间盘AQP1、AQP3表达的作用机制。方法：选取成年雄性新西兰大白兔50只,按照随机数字表法分为空白组（A组）、假模型组（B组）、模型组（C组）、模型＋电针组（D组）和模型＋电针＋阻断组（E组）,每组10只。建立椎体间压力诱导椎间盘退变模型后,A组、B组和C组不进行任何干预,D组行电针夹脊穴治疗,E组行电针夹脊穴治疗和PKA抑制剂H-89（10μmol/L）皮下注射,电针治疗6 d为1疗程,疗程间隔1 d,共治疗4个疗程。分别在造模成功后第1天和治疗后28 d两个时间点各取5只,进行椎间盘矢状位MRI T2扫描,采用Western-blot技术检测各组椎间盘组织内AQP1、AQP3的表达水平,ELISA方法检测椎间盘组织内cAMP水平及PKA活性。结果：造模成功后第1天,C组、D组、E组AQP1、AQP3表达及cAMP、PKA活性浓度明显下降,差异有统计学意义（P〈0.05）。治疗后第28天D组中AQP1、3的表达及cAMP、PKA活性浓度明显高于同期C组（P〈0.05）;E组中AQP1、AQP3表达及PKA活性浓度显著低于同期D组（P〈0.05）。Objective： To study the mechanism of cAMP-PKA signal pathway involved electroacupuncture（ EA） regulating the expressions of AQP1 and AQP3 in rabbits＇ disc degeneration. Methods： Fifty New Zealand white rabbits were randomly divided into 5 groups：normal group（ A group）,sham operation group（ B group）,model group（ C group）,model ＋ EA group（ D group）,model ＋ EA ＋ blockers group（ E group）,10 in each group. The disc degeneration model was established by using the external dynamic compression device attached to the rabbits＇ lumbar spine for 28 days. The model group recieved production of LIDD model without any treatment. The sham operation group recieved the same processing but without intervertebral pressure or any treatment. Model ＋ EA group recieved EA treatment since the 1 st day after model made. The model ＋ EA ＋ blockers group recieved the same processing as the model ＋ EA group and was injected PKA inhibitors（ H-89） at the 1 st day. A course for EA treatment lasted for 6 days. The total treatment had 4 courses. Between each of the course,1 day would be left. We took MRI test and tested dynamic expressions of AQP1,AQP3,cAMP and PKA at the 1 st day and 28 th day. Reslut： On the 1 st day after the model was set up,the AQP1,AQP3,cAMP and PKA expressions of C group,D group and E group were significantly decreased. On the 28 th day after treatment,the expressions of AQP1,AQP3,cAMP and PKA of D group were higher than those of the C group. The expressions of AQP1,AQP3,cAMP and PKA of E group were lower than those of D group. Conclusion： cAMP-PKA signal pathway involved in the EA increasing the expressions of AQP1 and AQP3 in the degenerated lumbar disc tissue.

关 键 词：电针 水通道蛋白1 水通道蛋白3 环磷酸腺苷 蛋白激酶A 信号传导 

分 类 号：R285.5[医药卫生—中药学]