当归补血汤对糖尿病大鼠肾组织内质网IRE1α-JNK通路的抑制作用  

Effect of Astragals and Angelica Mixture on Expression of IRE1α-JNK Pathway in Diabetic Nephropathy Rats

作　　者：帅瑜 张思泉[1] 沈鑫[1] 张莹雯[1] SHUAI Yu;ZHANG Siquan;SHEN Xin;ZHANG Yingwen(Department of TCM, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei, China)

机构地区：[1]武汉大学中南医院

出　　处：《中华中医药学刊》2018年第6期1372-1375,I0023,I0024,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81373793)

摘　　要：目的：为了研究当归补血汤对糖尿病肾病（diabetic nephropathy,DN）大鼠肾组织内质网应激通路IRE1α-JNK的作用机制及其对高糖下肾脏组织的保护作用。方法：链脲佐菌素（STZ）联合高脂饲料,建立糖尿病肾病动物模型,分为：正常对照组（N）、糖尿病肾病对照组（DN）、格列奇特组（GT）、当归补血汤低剂量组（DL）、当归补血汤高剂量组（DH）。对照组（N、DN）以生理盐水灌胃,治疗组分别以格列齐特缓释片溶液、高低浓度当归补血汤浓缩液灌胃处理8周后取材,用酶偶联比色法检测相关生化指标（血糖、血脂及肾功能指标）,光镜下观察肾脏病理改变;West Blog、RT-PCR检测IRE1α、JNK蛋白的表达,TUNEL法测定各组肾脏组织细胞的凋亡。结果：结果显示,DN组大鼠空腹血糖、血生化指标较N组明显升高;肾组织中p-IRE1α,p-JNK蛋白表达量较对照组N明显增加,细胞凋亡程度高;治疗组血糖、血脂、血肌酐等指标均低于DN组;肾脏p-IRE1α,pJNK蛋白表达较DN组下降;肾脏组织中细胞凋亡较DN组改善,N组与DH组无明显差异（P〉0.05）。结论：研究表明当归补血汤可以抑制高糖下肾组织IRE1α-JNK通路的表达,减轻高糖下肾脏的内质网应激反应,从而保护肾组织。Objective：To study the effect of Astragals and Angelica Mixture on IRE1 α -JNK signaling pathway related to endoplasmic reticulum stress in diabetic rats and explore its protection mechanisms. Methods ： Sixty rats induced with STZ except the NC group, fed with high -fat food, were randomly divided into five groups： blank group （N） , model group （DN）, gliclazide group（GT）, high and low dose of Astragals and Angelica Mixture groups （DtL/DL）. After 8 weeks, we tested the blood glucose,lipid, HDL and indicators related with renal function and the kidney structure changes were obserbed by optical microscope. Different expression levels of IRElα and JNK protection were tested after the opera- tion was performed by RT - PCR and Western blot. TUNELwas used to measure the apoptosis rate of each group. Results ： The DN group had a higher level of GLU, serumcreatinine and apoptosis rate than the N group. The protein expressions of p- IRElα and p- JNK increased significantly. Compared with DN group, the control groups （GT, DH and DL） had a lower level of GLU, serumcreatinine, apoptosis rate and the expressions of p- IREIα and p- JNK（P 〈0.05）. The GT and DH groups had no statistically significant difference （P 〉 O. 05）. Conclusions ： This study showed that Astragals and Angelica Mixture inhibited endoplasmic reticulum stress by restraining IREIα -JNK signaling pathway to protect the renal function.

关 键 词：当归补血汤 内质网应激 IRElα-JNK通路 糖尿病肾病 

分 类 号：R587.1[医药卫生—内分泌]