二草清肝汤对内毒素性肝损害模型大鼠肝脏Kupffer细胞TLR-4信号转导通路的影响  

Effect of Ercao Qinggan Decoction on TLR-4 Signaling Pathway in Kupffer of Acute Liver Injury of Rats with Endotoxemia

作　　者：刘三海 吴春明[1] 林上助[2] 欧阳钦[1] 黄强[1] 陈靓[1] LIU Sanhai;WU Chunming;LIN Shangzhu;OUYANG Qin;HUANG Qiang;CHEN Liang(Department of Liver Disease, The Traditional Chinese Medical Hospital of Wenzhou, Wenzhou 325000, Zhejiang, China;Department of Gastroenterology, The Traditional Chinese Medical Hospital of Wenzhou, Wenzhuu 325000, Zhejiang, China)

机构地区：[1]温州市中医院肝病科,浙江温州325000 [2]温州市中医院消化科,浙江温州325000

出　　处：《中华中医药学刊》2018年第6期1522-1525,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：温州市科技计划项目(Y20130306)

摘　　要：目的：探讨二草清肝汤对内毒素性肝损害模型大鼠肝脏Kupffer细胞的活化及功能依赖TLR4信号传导通路的影响。方法：120只雄性Wistar大鼠,随机分成空白组、模型组、二草清肝汤大剂量（20 g/kg）和小剂量组（10 g/kg）,每组30只,连续灌胃给药12 d。末次给药4 h后,除空白组外所有大鼠腹腔注射D-Gal N（800 mg/kg）/LPS（0.04 mg/kg）诱导内毒素性肝损害,造模后1、2、4、6、12、24 h每组取5只大鼠,Western印迹法检测Kupffer细胞Toll样受体-4（TLR-4）、TIR结构域的衔接蛋白（TRIF）、IL-1相关蛋白激酶（IRAK）、核因子-κB（NF-κB）蛋白表达变化。结果：造模后1、2 h,各组大鼠TLR-4、TRIF、IRAK、NF-κB蛋白表达无显著差异。造模后4、6、12、24 h,与空白组比较,模型组大鼠肝脏Kupffer细胞TLR-4、TRIF、IRAK、NF-κB蛋白表达明显增多（P〈0.05）;与模型组比较,二草清肝汤大、小剂量组大鼠肝脏Kupffer细胞TLR-4、TRIF、IRAK、NF-κB蛋白表达明显减少（P〈0.05）;大、小剂量组同时间比较无显著差异。结论：二草清肝汤可能通过抑制TLR-4信号转导通路的活性发挥其抗炎效应。Objective：To observe the effect of Ercao Qinggan Decoction on Toll like receptor -4 （TLR -4） signaling pathway in the acute liver injury of rats with endotoxemia. Methods：A total of 120 Wistar rats were randomized into 4 groups： normal control group, model group, high - dose of Ercao Qinggan Decoction group, and low - dose of Ercao Qinggan Decoction group, 30 in each. After 12 days of pretreatment, D -GaIN and LPS were intraperitoneally injected to the model and experimental groups. In 1,2,4,6,12 and 24 h after injection, 5 rats in each group, the protein expressions of TLR - 4, TRIF, IRAK and NF - KB in the Kupffer of liver were tested by Western - Blot technique. Results ： There was no statistical significance in the protein expressions of TLR - 4, TRIF, IRAK and NF - KB among groups in 1 and 2 h of administration （ P 〉 0.05 ）. In 4,6,12 and 24 h, the protein expressions of TLR - 4, TRIF, IRAK and NF - KB increased significantly in the model group as compared with those of the blank group（ P 〈 0.05）. The protein expressions of TLR - 4,TRIF, IRAK and NF - KB decreased significantly in the two medication groups as compared with those of the model group（P 〈 0.05）. The difference between two medication groups was not statistically significant at the same time period （ P 〉 O. 05 ）. Conclusion ： Ercao Qinggan Decoction may has an anti - inflammatory effect by inhibiting the activity of TLR -4 signaling pathway.

关 键 词：二草清肝汤 内毒素性肝损害 大鼠 KUPFFER细胞 TLR-4通路 

分 类 号：R285.5[医药卫生—中药学]