三种丛枝菌根真菌对茅苍术的生长、生理及主要挥发油成分的影响  

Effects of three arbuscular mycorrhizal fungi(AMF) species on the growth,physiology,and major components of essential oil of Atractylodes lancea

作　　者：梁雪飞 唐梦君 吕立新 赵翔宇 戴传超 LIANG Xue-fei;TANG Meng- jun;LV Li-xin;ZHAO Xiang-yu;DAI Chuan-chao(Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources, Jiangsu Key Laboratory for Microbes and Functional Genomics, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China）.)

机构地区：[1]南京师范大学生命科学学院江苏省微生物与功能基因组学重点实验室江苏省微生物资源产业化工程技术研究中心,南京210023

出　　处：《生态学杂志》2018年第6期1871-1879,共9页Chinese Journal of Ecology

基　　金：南京市科委产学研合作项目(201306019);江苏高校优势学科建设项目;国家自然科学基金项目(31070443)资助

摘　　要：在温室条件下研究了3种丛枝菌根真菌(arbuscular mycorrhizal fungus,AMF):Glomus etunicatum BGC AM0048(A1)、Glomus tortuosum BGC AM0001(A2)和Glomus mosseae BGC AM0045(A3)对茅苍术组培苗的成活率、生长、生理特性及挥发油积累的影响。结果表明:接种AMF均提高了茅苍术组培苗的移栽成活率;苍术苗移栽35 d后,3个接种组的菌根侵染率由高至低依次为A3(63.39%)、A2(47.81%)和A1(42.41%);菌株对苍术苗的生长促进作用与侵染率规律一致,以A3对苍术苗的生长促进作用最明显,苍术苗的单株株高、单株根长、单株叶片数较对照组分别增加了21.9%、65.%和38.7%,各项检测指标与对照组的差异均达到显著水平;AMF处理组叶片中抗病相关酶系(PAL、PPO和β-1,3-葡聚糖酶)、抗氧化酶系(SOD、CAT和POD)的活性和叶绿素含量均有不同程度的提高,且接种AM真菌均促进了苍术苗挥发油中3种主要成分苍术醇、β-桉叶油醇和苍术素的积累。不同的AMF与植物之间具有偏好性,在人工栽培中,G.mosseae BGC AM0045菌株可作为茅苍术苗菌根化处理的优良菌株。To investigate the effects of arbuscular mycorrhizal fungi（ AMF） on survival,growth,physiological characteristics,and essential oil accumulation of Atractylodes lancea plantlets,three strains of AMF,including Glomus etunicatum BGC AM0048（ A1）,Glomus tortuosum BGC AM0001（ A2）,and Glomus mosseae BGC AM0045（ A3）,were used to inoculate A. lancea tissue culture seedlings in a greenhouse experiment. The results showed that the inoculation of AMF increased the survival rate of seedlings. The mycorrhizal infection rates in the three inoculated groups were in the order of A3（ 63. 39%）,A2（ 47. 81%）,A1（ 42. 41%） at 35 d after transplanting,and their positive effects on the growth of A. lancea followed the same order of infection rate,with A3 group showing the best performance. Compared to the control group,plant height,root length,and leaf number per plant in A3 group significantly increased by 21. 9%,65% and 38.7% respectively. The activities of disease resistance enzymes（ PAL,PPO,β-1,3-glucanase）,antioxidant enzymes（ SOD,CAT and POD） as well as chlorophyll（ a ＋b） content in the leaves of AMF treatment groups were improved at some extent. Moreover,AMF inoculation increased the accumulation of the major components（ hinesol,β-eudesmol and atractylodin） in the essential oil. Our results showed that there was difference in the preference of AMF for plants.G. mosseae BGC AM0045（ A3） could be used as an excellent fungal strain to inoculate A. lancea seedlings for the establishment of arbuscular mycorrhizae in artificial cultivation.

关 键 词：球囊霉属 茅苍术组培苗 共生偏好性 

分 类 号：S567.239[农业科学—中草药栽培]