花生HyPGIP基因克隆及抗叶腐病表达分析  

Cloning and expression analysis of HyPGIP gene related to peanut leaf rot resistance

作　　者：隋鹏飞 王麒然 王琰 王志葵 张茹琴[1] 迟玉成[4] 夏淑春[1] 鄢洪海[1] SUI Peng-fei;WANG Qi-ran;WANG Yan;WANG Zhi-kui;ZHANG Ru-qin;CHI Yu-cheng;XIA Shu-chun;YAN Hong-hai(College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China;Shandong Rushan City Agriculture Bureau, Weihai 264500, China;Jimo Agriculture Bureau, Qingdao 266200, China;Peanut Research Institute in Shandong Province, Qingdao 266101 ,China)

机构地区：[1]青岛农业大学农学与植物保护学院,青岛266109 [2]山东省乳山市农业局,威海264500 [3]青岛市即墨市农业局,青岛266200 [4]山东省花生研究所,青岛266101

出　　处：《植物病理学报》2018年第3期346-356,共11页Acta Phytopathologica Sinica

基　　金：山东省科技发展项目(2009GG10009022);山东省”泰山学者”建设_[程专项经费资助(BS2009NY040)

摘　　要：本文采用基因克隆和生物信息学方法研究了花生Hy PGIP基因及编码蛋白的基本性质和生物学功能,并通过测定接种叶腐病菌后花生植株内PG酶的活性变化和PGIP蛋白的生成量变化,以及Hy PGIP基因瞬时表达分析,探讨了PGIP蛋白与寄主抗病性关系。结果表明,受叶腐病菌侵染的花生植株中,抗病品种的PG酶活性都明显低于感病品种,而生成的PGIP蛋白量都比感病品种显著多,暗示PGIP蛋白与花生叶腐病抗性有关。经基因克隆和测序获得HyPGIP基因全长序列为1 029 bp,编码342个氨基酸,无内含子,终止密码子为TGA,与已报道的五个花生PGIP序列的同源性都很高;结构预测发现该序列存在8个LRR结构域,存在信号肽,疏水性较强,定位于细胞壁上;RT-PCR显示,经接种叶腐病菌处理后,花生植株中的PGIP基因表达量明显增加。In this paper,the basic and biological functions of HyPGIP gene and its encoded protein were studied by gene cloning and bioinformatics methods,and the relationship between PGIP protein and host disease resistance was explored by determining PG enzyme activity,PGIP protein content and analyzing transient expression of HyPGIP gene in peanut plants after inoculation with Rhizoctonia solani. The results indicated that,PG activity in the resistant cultivars was significantly lower than that of in the susceptible cultivars,while the PGIP protein content in the resistant cultivars was significantly higher than that of in the susceptible cultivars,this result suggested that PGIP protein was related to peanut rot resistance. The full-length sequence of HyPGIP gene was 1 029 bp,encoding 342 amino acids,no intron,and the termination codon was TGA,which was highly homologous to the reported 5 PGIP sequences. There were 8 LRR domains in the sequence,and there were signal peptides,stronger hydrophobicity and located on the cell wall. RT-PCR showed that PGIP gene expression in peanut plants significantly increased after inoculation with R. solani.

关 键 词：花生叶腐病 多聚半乳糖醛酸酶(PG) 多聚半乳糖醛酸酶抑制蛋白(PGIP) HyPGIP基因克隆 定量分析 

分 类 号：S432.44[农业科学—植物病理学]