帕瑞昔布钠对脓毒症大鼠肠黏膜屏障功能的影响  

Effects of parecoxib on intestinal mucosa barrier in a rat model of sepsis

作　　者：刘志慧[1] 于泳浩[2] 李佩铂[1] 武云飞[1] 田义川 Liu Zhihui;Yu Yonghao;Li Peibo;Wu Yunfei;Tian Yichuan(Department of Anesthesiology, Baotou Central Hospital, Baotou 014040, China;Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin 300052, China)

机构地区：[1]包头市中心医院麻醉科,014040 [2]天津医科大学总医院麻醉科,300052

出　　处：《国际麻醉学与复苏杂志》2018年第5期395-399,共5页International Journal of Anesthesiology and Resuscitation

基　　金：内蒙古自治区自然科学基金（2017BS0802）

摘　　要：目的 探讨帕瑞昔布钠对脓毒症大鼠肠屏障功能的影响。 方法 采用盲肠结扎穿孔法（cecal ligation and puncture, CLP）诱导脓毒症大鼠肠损伤模型。72只Wistar大鼠按随机数字表法分为4组（每组18只）：假手术组（Sham组）、假手术＋10 mg/kg帕瑞昔布钠组（SP组）、脓毒症组（CLP组）、脓毒症＋10 mg/kg帕瑞昔布钠组（CP组）。SP组和CP组大鼠于假手术或CLP后20 min腹腔注射帕瑞昔布钠10 mg/kg，12 h后再重复注射1次。CLP组和Sham组大鼠仅经腹腔注射等量生理盐水。于假手术或CLP后24 h，检测血浆二胺氧化酶（diamine oxidase, DAO）和D-乳酸的浓度，检测各组大鼠肠组织紧密连接蛋白（zonula occludens-1, ZO-1）和Claudin-1的蛋白表达，检测肠组织髓过氧化物酶（myeloperoxidase， MPO）的活性水平。光镜检测肠组织的病理学变化。 结果 与CLP组比较，CP组脓毒症大鼠血浆中DAO和D-乳酸水平降低（P〈0.05），MPO活性下降（P〈0.05），CP组肠组织ZO？蛳1和Claudin-1表达上调（P〈0.05），CP组Chiu＇s评分降低（P〈0.05）。 结论 10 mg/kg帕瑞昔布钠治疗脓毒症大鼠能够减轻肠组织损伤和炎症反应，有效降低肠黏膜的通透性，改善肠屏障功能。Objective To explore the effects of parecoxib on the intestinal mucosa barrier in a mouse model of sepsis. Methods Cecal ligation and puncture procedure （CLP） was applied to induce sepsis. Seventy-two Wistar rats were randomly divided into 4 groups （n=18）： Sham, Sham＋10 mg/kg parecoxib （SP）, CLP, CLP＋10 mg/kg parecoxib （CP）. Rats were intraperitoneally injected with 10 mg/kg parecoxib or same volume of saline 20 min after CLP or sham operation, twice a day. Twenty-four hours after operation, the activity of diamine oxidase （DAO）, D-lactate, and intestinal tissue myeloperoxidase （MPO） was measured. The levels of intestinal tight junction protein including Claudin-1 and zonula occludens-1（ZO-1） were detected. The pathological alterations in the intestine were revealed with microscopy. Results Parecoxib treatment reduced the activity of MPO in the intestinal tissue, decreased the plasma level of DAO and D-lactate, and significantly attenuated the damage of the intestinal tissue（P〈0.05 vs group CLP）. Meanwhile, Parecoxib upregulated the expression of ZO-1 and Claudin-1 protein which may be associated with the homeostasis of the intestinal mucosa barrier（P〈0.05 vs group CLP）. Conclusions Parecoxib treatment improveds the intestine injury, and reduced the permeability of the intestinal mucosa barrier in sepsis.

关 键 词：脓毒症 帕瑞昔布钠 肠黏膜屏障 

分 类 号：R614[医药卫生—麻醉学]