有机玻璃刻痕增强彗星电泳的凝胶粘附能力  

Nicking plexiglass enhancingadhesion ability ofgel in comet assay

作　　者：方佳宁 张回 孔令东 王攀攀 何文涓 陈功星[1] FANG Jianing;ZHANG Hui;KONG Lingdong(Holistic Integrative Pharmacy Institutes of Hangzhou Normal University, Hangzhou 310036, China;Wuxi Higher Health Vocational Technology School, Wuxi 214028, China)

机构地区：[1]杭州师范大学整合药学院,浙江杭州310036 [2]无锡卫生高等职业技术学校,江苏无锡214028

出　　处：《华夏医学》2018年第1期31-33,共3页Acta Medicinae Sinica

基　　金：杭州师范大学本科生课外科研项目(CX2016143;5;6);江苏省卫计委资助课题(J201616);浙江省基础公益研究计划资助项目(LGF18H290002)

摘　　要：目的:增强凝胶在支持物上的粘附能力,防止彗星电泳时脱胶。方法:Jurkat细胞株与凝胶液混和,涂抹专用检测载玻片和刻痕有机玻璃片,然后进行常规彗星凝胶电泳,荧光显微镜下分析DNA损伤。结果:刻痕有机玻璃片防脱胶率96.8%,与专用试剂盒载玻片没有统计学差异(P>0.05),刻痕有机玻璃片不影响电泳效果。结论:刻痕有机玻璃可用来进行彗星电泳,检测细胞DNA损伤。Objective： To enhance the adhesion of gelatin on support and prevent degumming during comet electrophoresis. Methods： The Jurkat cell line was mixed with the gel,and the mixture was transferred to the special slide glass and the nicking plexiglass. Then,the conventional comet gel electrophoresis was carried out,and the DNA damage was analyzed under the fluorescence microscope. Results： The antidegumming rate of the nicking plexiglas was 96.8%,and there was no statistical difference from that of the special slide glass（ P 〉 0. 05）, and the nicking plexiglas did not affect the electrophoretic effect.Conclusion： Nicked plexiglass can be used for comet electrophoresis to detect DNA damage in cells.

关 键 词：有机玻璃 刻痕 粘附能力 彗星电泳 

分 类 号：Q71[生物学—分子生物学]