绞股蓝皂苷抑制AGEs诱导的人肾小球系膜细胞TGF-β1及PDGF表达  

Gypenosides inhibit AGEs induced TGF-beta 1 and PDGF expression in human glomerular mesangial cells

作　　者：张慧云[1] 唐灵[1] 张秋艳 徐庆博 ZHANG Huiyun;TANG Ling;ZHANG Qiuyan;et al(The Affiliated Hospital of Guilin Medical University, Guilin 541001, China;Zhengzhou Orthopaedics Hospital, Zhengzhou 450052, China)

机构地区：[1]桂林医学院附属医院,广西桂林541001 [2]郑州市骨科医院,河南郑州450052

出　　处：《华夏医学》2018年第1期50-53,共4页Acta Medicinae Sinica

基　　金：广西壮族自治区卫生和计划生育委员会课题(Z2016387)

摘　　要：目的:探讨绞股蓝皂苷(GP)对晚期糖基化终末产物(AGEs)诱导的人肾小球系膜细胞(human glomerular mesangial cells,HMCs)TGF-β1及PDGF表达的抑制作用。方法:采用AGEs诱导HMCs细胞TGF-β1及PDGF表达,采用不同剂量GP细胞72 h后,RT-PCR和Western blotting检测TGF-β1、PDGF的表达。结果:AGEs能诱导HMCs中TGF-β1、PDGF表达。GP对诱导TGF-β1、PDGF表达有抑制作用,呈浓度越高,抑制作用越强。结论:GP可通过抑制TGF-β1、PDGF表达改善糖尿病肾病病情。Objective： To investigate the inhibitory effect of Gynostemma Pentaphyllum（ GP） on the expression of human glomerular mesangial cells（ HMCs） TGF-beta 1 and PDGF induced by advanced glycation end product（ AGEs）. Methods： The expression of TGF-beta 1 and PDGF in HMCs cells was induced by AGEs. After 72 h incubation with different doses of GP,RT-PCR and Western blotting were used to detect the expression of TGF-beta 1 and PDGF. Results： AGEs can induce the expression of TGFbeta 1 and PDGF in HMCs. GP inhibited the expression of TGF-beta 1 and PDGF. The higher the concentration,the stronger the inhibition was. Conclusion： GP can improve the condition of diabetic nephropathy by inhibiting the expression of TGF-beta 1 and PDGF.

关 键 词：绞股蓝皂苷 糖尿病肾病 晚期糖基化终产物 转化生长因子-β1 血小板衍化生长因子 

分 类 号：R587.1[医药卫生—内分泌]