1株田间自然重组PRRSV类NADC30毒株的分离鉴定及序列分析  

Isolation,identification and sequence analysis of a natural recombinant PRRSV NADC30-like strain

作　　者：崔丹丹[1] 彭志锋[1,2] 王傲杰 王新港 周峰[1] 王川庆[1] CUI Dan-dan;PENG Zhi-feng;WANG Ao-jie;WANG Xin-gang;ZHOU FengI;WANG Chuan-qing(College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002,China;？. Department of Animal Medicine, Henan Institute of Livestock Econora ics , Zhengzhou 450046, Chin)

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南牧业经济学院动物医学院,河南郑州450046

出　　处：《中国兽医学报》2018年第6期1071-1076,共6页Chinese Journal of Veterinary Science

基　　金：河南省高校科技创新团队与支持计划资助项目(14IRTSHN015);预防兽医重点学科资助项目(MXK2016102)

摘　　要：为了解田间猪繁殖与呼吸综合征病毒（PRRsv）的流行及变异情况，本研究将PRRSV阳性样品接种到Marc－145细胞并传代后成功分离到1株PRRSV，将其命名为HENZMD-9，并对其全基因序列进行测定和分析。结果显示，HENZMD-9为1株类美国NADC30毒株，并与国内高致病性PRRSV（HP—PRRRSV）毒株的2个片段发生重组，这2个片段分别来自HP—PRRSV JXA1株的细胞传代致弱株JXA1-P80及JXA1P45，分别位于基因组5’端及开放式阅读框lab区域，表明HENZMD-9很有可能与JXA1-R疫苗回复株发生了重组，提示不同类型毒株在田间的共存会加剧PRRSV的演变，从而增加临床防控难度。因此，持续监测PRRSV田间流行及变异动态对PRRSV的防控具有一定的，临床指导意义。To understand the prevalence and molecular variation characteristics of porcine reproduc tive and respiratory syndrome virus （PRRSV）in the field, a PRRSV positive sample was isolated and inoculated onto the Marc-145 cell layers and developed by serial passage,then a strain was ob- tained neamed HENZMD-9. The whole genome was then sequenced and analyzed, which showed that HENZMD-9 was a NADC30-1ike strain recombined with highly pathogenic PRRSV （HP- PRRRSV）. There existed two substitution fragments which were from the attenuated strains JXA1-PS0 and JXA1-P45 developed by serial passage of HP-PRRSV strain JXA1-R in Marc-145 cells,and were located in the 5rend and ORFlab of the genome,respectively. These results indica- ted that HENZMD 9 was most probably recombined with the revertants of the vaccine strain JXA1-R,warning that coexistence of different kinds of PRRSVs in the fields can aggravate the evolution of PRRSV and thus increase the difficulties in clinical prevention and control. Therefore, continuously monitoring the prevalence and dynamic variation of predominant PRRSVs in the fields has certain clinically guiding significance on PRRSV prevention and control.

关 键 词：猪繁殖与呼吸综合征病毒 分离鉴定 序列分析 重组分析 类NADC30株 JXA1-R疫苗回复突变株 

分 类 号：S852.65[农业科学—基础兽医学]