亚低温冷刺激对大鼠肝细胞中SOD、CAT和Caspase-3的影响  

Effects of mild hypothermia on SOD,CAT and Caspase-3 in rat hepatocytes

作　　者：薛琳琳[1] 付云超[1] 邹洪波[1] 郭文晋[2] 杨焕民[2] XUE Lin-lin;FU Yun-chao;ZOU Hong-bo;GUO Wen-jin;YANG Huan-min(Heilongjiang Polytechnic, Harbin 150000, China;Heilongiang Bayi Agricultural University, Daqing , Heilongjiang 163000 ,China)

机构地区：[1]黑龙江职业学院,黑龙江哈尔滨150000 [2]黑龙江八一农垦大学,黑龙江大庆市163000

出　　处：《中国兽医学报》2018年第6期1168-1170,共3页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31372398);十三五国家重点研发计划子课题资助项目(2016YFD0501210)

摘　　要：为研究冷应激状态下大鼠肝细胞系的抗氧化水平，本试验选取3个时间点（0，4，8h）分别对大鼠肝细胞系进行32℃冷刺激，并分别对3个时间点细胞的超氧化物歧化酶、过氧化氢酶和Caspase-3的活性进行检测。结果发现，32℃冷刺激时，超氧化物歧化酶和过氧化氢酶的活性并无显著性变化，但有下降的趋势，而Caspase3在4h组的含量显著高于0，8h组（P〈0.05），8h纽的含量显著高于0h组（P〈0.05）。结果表明，冷应激很有可能对大鼠肝细胞系的抗氧化水平产生不利影响，从而引起细胞出现一系列异常变化，甚至会导致肝细胞凋亡，影响正常的肝细胞功能。For the study on cold stress state antioxidant levels in rat liver cell line,in this experiment,three time points（0 h,4 h and 8 h）were selected for cold stimulation to the rat liver cell lines at 32℃ ,and then the ceils superoxide dismutase（sod）,catalase and the activity of Caspase 3 were inspected. The result showed that there was no significant change in the activity of superoxide dismutase（SOD） and catalase,but have a downward trend. The content of Caspase 3 in 4 h group was significantly higher than that of 0 and 8 h group（P〈0.05）. The content in 8 h group was signifi cantly higher than that of 0 h group（P〈0.05）. The experimental results showed that cold stress may be have the adverse effects on level of antioxidant of rat liver cell lines, causing liver cell apop- tosis,and normal function.

关 键 词：大鼠肝细胞 冷应激 过氧化氢酶 超氧化物歧化酶 CASPASE-3 

分 类 号：S831.1[农业科学—畜牧学]