SPE-HPLC法同时测定汉麻提取物中3种大麻酚含量  

Determination of Three Cannabinoids in Hemp Extraction by SPE-HPLC

作　　者：张旭[1] 孙宇峰[1] 田媛[1] 石雨[1] 崔宝玉[1] 高宝昌[1] ZHANG Xu;SUN Yu-feng;TIAN Yuan;SHI Yu;CUI Bao-yu;GAO Bao-chang(Daqing Branch of Heilongjiang Academy of Sciences, Daqing 163319, China)

机构地区：[1]黑龙江省科学院大庆分院

出　　处：《化学试剂》2018年第6期547-550,554,共5页Chemical Reagents

基　　金：黑龙江省科学院青年创新基金面上项目(CXMS2017DQ03);国家国际科技合作专项项目(2015-DFR50260)

摘　　要：建立了SPE-HPLC法同时测定3种大麻酚的方法。液相检测条件：Venusil XBP C18（L）：4.6×150 mm,5μm;流动相为V（甲醇）∶V（0.1%甲酸）=80∶20;柱温为40℃;检测波长为220 nm;流速为1 m L/min。3种大麻酚的线性回归方程R2均大于0.999,精密度实验、重复性实验RSD均在1.05%以内,回收率范围为99.6%-112.7%。此方法操作简便、准确度高,可作为3种大麻酚的质量控制标准。To establish a SPE-HPLC method for three cannabinoids.The conditions of HPLC contain Venusil XBP C18（ 4. 6×150 mm,5 μm） column and the mobile phase with methanol of 80% and 0. 1% formic acid of 20%. The column temperature was40 ℃ and the cannabinoids were detected at 220 nm.The flow rate was 1 m L/min.The recoveries are greater than 0. 999.The RSD of precision and repeatability are less than 1. 05%.The recoveries are between 99. 6% and 112. 7%.This method is easy to operate and high accuracy,it can be used as quality control standards of three cannabinoids.

关 键 词：汉麻提取物 SPE-HPLC法 大麻酚 

分 类 号：O657.7[理学—分析化学]