深度覆盖的蛋白质组精准鉴定与定量新技术  

Novel Technologies For Deep-Coverage and Precise Proteome Identification and Quantification

作　　者：张丽华[1] 单亦初[1] 张弓[2] 张养军[3] 张祥民[4] Zhang Lihua;Shan Yichu;Zhang Gong;Zhang Yangjun;Zhang Xiangmin(Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023;Jinan University, Guang Zhou 510632;Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850;Fudan University, Shanghai 200433)

机构地区：[1]中国科学院大连化学物理研究所,大连116023 [2]暨南大学,广州510632 [3]中国人民解放军军事医学科学院放射与辐射医学研究所,北京100850 [4]复旦大学,上海200433

出　　处：《中国基础科学》2018年第1期49-52,共4页China Basic Science

基　　金：国家重点研发计划蛋白质机器与生命过程调控专项项目(2017YFA0505000)

摘　　要：蛋白质,尤其是低丰度蛋白质,种类和含量的变化在调控生命过程中发挥着至关重要的作用。因此深度覆盖的蛋白质组精准鉴定与定量新技术对深入认识蛋白质机器的动态变化规律具有重要意义。针对目前蛋白质组定性定量分析领域存在的可变剪切和新生肽链组鉴定灵敏度低、蛋白质组精准定量覆盖度低以及纯化蛋白质全序列测定准确度低等关键科学问题,通过发展可变剪切和新生肽链组的高灵敏鉴定技术、基于高效标记和特征肽段的蛋白质组精准定量技术、基于高效分离的蛋白质组深度覆盖定量技术以及纯化蛋白质的全序列高准确测定技术等具有原始创新性的新一代蛋白质组学分析技术,显著提高蛋白质组分析的灵敏度、精准度和覆盖度,并将发展的新技术示范性用于与生物医药、国家安全和人口健康领域密切相关的耐药菌、耐辐射球菌、人肝癌高低转移细胞株等的深度覆盖蛋白质组精准定量分析,进而为满足国家重大领域的实际需求提供关键技术支撑。Variety and expression level change of proteins, especially low abundance proteins, play a vital role in the regulation of life processes. Therefore, it is very important to develop novel technologies for deep coverage and precise proteome identification and quantification in order to deeply understand the dynamic change of protein machines. To address the key scientific issues such as low detection sensitivity for alternative splicing and nascent peptide chains, low coverage of precise proteome quan- tification and low accuracy for full-length sequencing of purified proteins, new generation of technologies for proteomics analysis including high sensitive identification of alternative splicing and nascent peptide chains, precise quantification of proteome based on high effi- cient labeling and characteristic peptides, deep coverage proteome quantification based on high efficient separation and high accuracy full-length sequencing of purified proteins will be developed, which will significantly improve the sensitivity, precision and coverage of proteome analysis. The developed novel technologies will be further applied in the deep coverage and precise proteome quantification of drug-resistant bacteria, radiation-resistant cocci, high and low metastatic cell strains of human hepatocellular carcinoma which are closely related to the areas of bio-medicine, national security and population health and provide key technical support to meet the real needs of the country＇s major areas.

关 键 词：蛋白质组学 深度覆盖 精准定量 蛋白质测序 

分 类 号：Q503[生物学—生物化学]