1,4-二氢吡啶衍生物的合成及其与牛血清白蛋白的相互作用  

Syntheses of 1,4-Dihydropyridine Derivatives and Their Interaction with Bovine Serum Albumin

作　　者：洪利明 唐爱清 谢晓敏 张志兰 Hong Liming;Tang Aiqing;Xie Xiaomin;Zhang Zhilan(College of Chemistry and Environmental Science, Shangrao Normal University, Shangrao 334001)

机构地区：[1]上饶师范学院化学与环境科学学院,上饶334001

出　　处：《化学通报》2018年第6期537-542,共6页Chemistry

基　　金：江西省自然科学基金项目(20151BAB203046);校级大学生创新训练项目(2017-CX-82)资助

摘　　要：以对甲苯磺酸为催化剂,一锅法合成了一系列1,4-二氢吡啶衍生物,并对其进行了红外光谱、质谱和核磁共振表征。采用荧光光谱法、紫外光谱法对2,6-二甲基-4-苯乙烯-1,4-二氢吡啶-3,5-二甲酸乙酯(1a)与BSA在不同温度的相互作用进行了研究。在298K时,Kq为2.43×10^(12)L·mol^(-1)·s^(-1),Ka为4.15×10~3L·mol^(-1),n为0.82,ΔS为65.12J·mol^(-1)·K^(-1),ΔH为-1.24k J·mol^(-1)。研究表明,1a对BSA具有荧光猝灭作用,且1a与BSA的主要作用力可能是疏水作用力(ΔS>0)。1,4-Dihydropyridine derivatives were synthesized by one-pot methods using p-toluene sulfonic acid as catalyst,and characterized by IR,MS and ~1 H NMR. The interaction of the compound 1 a with bovine serum albumin（ BSA） was studied using fluorescence and UV-Vis spectroscopy at different temperatures. Experimental results indicated that Kq,Ka,n,ΔS,ΔH were 2. 43 × 10^（12） L·mol^-1·s^-1,4. 15 × 10~3 L·mol^-1,0. 82,65. 12 J·mol-1·K-1 and 1. 24 k J·mol^-1,respectively,at 298 K. The results showed that the compound 1 a has a fluorescence quenching effect on BSA. Parameters of thermodynamic functions were calculated,and the interaction between BSA and 1 a is determined as hydrophobic interaction.

关 键 词：牛血清白蛋白 荧光猝灭 1 4-二氢吡啶衍生物 疏水作用力 

分 类 号：TQ460.1[化学工程—制药化工]