合欢皮对小鼠焦虑行为影响及抗焦虑活性部位筛选  

Effect of Cortex Albiziae on Mice's Anxiety-like Behavior and Antianxiety Active Site Screening

作　　者：熊永豪 冯波[1] 牛源菲 崔瑛[1] 王君明[1] 王红丽[1] 管俊[1] Xiong Yonghao;Feng Bo;Liu Yuanfei;Cui Ying;Wang Junming;Wang Hongli;Guan Jun(Henan University of Chinese Medicine, Zhengzhou 450046, Chin)

机构地区：[1]河南中医药大学,郑州450046

出　　处：《世界中医药》2018年第4期790-793,798,共5页World Chinese Medicine

基　　金：郑州市科技领军人才项目(121PLJRC534)

摘　　要：目的:研究合欢皮对小鼠焦虑行为的影响,并对其活性部位进行初步筛选。方法:采用高架十字迷宫、明暗箱实验对合欢皮水提液、醇提液及各萃取部位进行抗焦虑研究;采用酶联免疫法检测脑内γ-氨基丁酸(GABA)、谷氨酸(Glu)和5-羟色胺(5-HT)含量。结果:高架十字迷宫、明暗箱实验显示,合欢皮水提液各剂量组具有明显的抗焦虑作用(P<0.05),合欢皮醇提液有较弱的抗焦虑作用。合欢皮水提液正丁醇萃取组及合欢皮醇提液正丁醇萃取组具有明显的抗焦虑作用(P<0.05);酶联免疫实验结果显示,合欢皮水提液、合欢皮水提液正丁醇萃取组及合欢皮醇提液正丁醇萃取组均能明显提高小鼠脑内GABA含量,降低小鼠脑内Glu含量,降低小鼠脑内5-HT含量。结论:合欢皮对小鼠具有显著的抗焦虑作用;其作用机制与提高小鼠脑内GABA含量,降低小鼠脑内Glu和5-HT含量含量有关;其发挥抗焦虑作用的活性部位主要为正丁醇部位。Objective： To study the anxiolytic effects of Cortex Albiziae on mice,and make a preliminary screening of its active site. Methods： We studied the anti-anxiety effect of water extraction of Albizia,alcohol extraction and various parts of Albizia extraction by the elevated plus maze,light-dark. The contents of GABA,Glu,5-HT in brain were detected by ELISA. Results： The results of elevated plus maze and light-dark experiments showed that each dose group of water extraction of Cortex Albiziae had a significant anxiolytic effect（ P 〈 0. 05）,and each dose group of Cortex Albiziae alcohol extract didn＇ t show a significant anxiolytic effect（ P 〉 0. 05）. Cortex Albiziae water extract butanol group and Cortex Albiziae alcohol extract butanol group had clearly anxiolytic effect（ P 〈 0. 05）; ELISA results showed that water extract of Cortex Albiziae,Cortex Albiziae extract butanol group and Cortex Albiziae alcohol butanol extract could significantly increase the content of GABA in mice,reduce Glu and 5-HT content in brain. Conclusion： Cortex Albiziae has a significant anxiolytic effect on mice. And this mechanism is related with its effects of improving GABA content and reducing Glu and 5-HT content in the brain of mice. The anxiolytic activity site of Cortex Albiziae is the n-butanol fraction.

关 键 词：合欢皮 抗焦虑 高架十字迷宫 明暗箱 正丁醇部位 

分 类 号：R285.5[医药卫生—中药学]