生防菌解淀粉芽胞杆菌Jt84干悬浮剂加工及田间评价  

Evaluation of Bacillus amyloliquefaciens Jt84 Dry Flowable Formulation Production and Control Effect against Rice Blast in Field

作　　者：张荣胜[1] 王法国[1] 齐中强[1] 杜艳[1] 刘永锋[1] ZHANG Rongsheng;WANG Faguo;Qi Zhongqiang;DU Yan;LIU Yongfeng(Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014

出　　处：《中国生物防治学报》2018年第3期407-413,共7页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2017YFD0201100);国家"948"项目(2016-X48);江苏省自主创新资金(CX(17)3021)

摘　　要：解淀粉芽胞杆菌Jt84菌株对水稻真菌病害具有良好的防治效果。本研究通过喷雾干燥技术,开发一种生防菌新剂型杀菌剂。采用Plackett-Burman试验及Box-Behnken试验,对解淀粉芽胞杆菌Jt84干悬浮剂喷雾干燥工艺进行了优化。结合生产实际确定干悬浮剂制备条件分别为:进风温度118.9℃,进样速率25.7 r/min(约为680 mL/h),热风流量28.56 M^3/h,压力0.2 MPa,出风温度80℃,喷头口径0.7 mm。经喷雾干燥试验验证该理论预测值与实际值无显著差异。田间药效试验表明:水稻破口期喷施解淀粉芽胞杆菌Jt84干悬浮剂75 g/667 m^2,对水稻穗颈瘟的防治效果为79.3%,防治效果与三环唑相当,能够有效防治稻瘟病。Dry flowable formulations of Bacillus amyloliquefaciens Jt84 with fungicidal activity against the rice blast fungus Magnaporthe oryzae were developed by spray drying. The Plackett-Burman experiments and response surface methodology were applied to optimize the spray drying technology of B. amyloliquefaciens Jt84. According to the evaluation by the response surface model established in this study, the optimum conditions were inlet air temperature of 118.9℃, feed flow rate of 25.7 r/min （680 mL/h）, atomization caliber of 0.7 mm, atomization pressure of 0.2 MPa, hot air flow of 28.56 M3/h, and outlet air temperature of 80℃. Field trials showed that control efficacy of B. amyloliquefaciens Jt84 dry flowable formulation at 75 g/667 m^2 against rice blast was 79.3% at rupturing stage of rice, equal to that of 75% tricyclazole wettable powder.

关 键 词：解淀粉芽胞杆菌 喷雾干燥 稻瘟病 干悬浮剂 田间评价 

分 类 号：S476[农业科学—农业昆虫与害虫防治]