2015年烟台地区手足口病监测中柯萨奇病毒A4基因特征分析  

Characteristics of the Coxsackievirus A4 Gene in Surveillance for Hand,Foot and Mouth Disease in Yantai,China,in 2015

作　　者：孙振璐[1] 徐迎春[1] 刘娟[1] 高巧[1] 董兆静 刘虹 宫连凤 SUN Zhenlu;XU Yingchun;LIU Juan;GAO Qiao;DONG Zhaojing;LIU Hong;GONG Lianfeng(Yantai Center for Disease Control and Prevention, Yantai 264003, China;Yantai Infectious Diseases Hospital, Yantai 264000,China)

机构地区：[1]烟台市疾病预防控制中心,烟台264003 [2]烟台市传染病医院,烟台264000

出　　处：《病毒学报》2018年第4期471-476,共6页Chinese Journal of Virology

基　　金：2017-2018年度山东预防医学会智飞疾病预防控制技术研究基金项目计划(项目号:LYH2017-26);题目:引起手足口病大规模流行的CV-A4、CV-A6和CVA-10的进化~~

摘　　要：研究2015年山东省烟台地区手足口病的病原谱,以及柯萨奇病毒A组4型（Coxsackievirus A4,CV-A4）的分子流行病学。采用实时荧光逆转录聚合酶链反应方法,对2015年烟台地区采集于手足口病患者的696份样品进行总肠道病毒（EV）、柯萨奇病毒A16（CV-A16）型和肠道病毒A71型（EV-A71）的核酸检测,并对非EV-A71非CV-A16的EV阳性标本,进行CV-A4的核酸检测。对CV-A4阳性标本进行病毒分离,对病毒分离阳性的CV-A4标本进行全长VP1区扩增,并进行核苷酸序列测定和分析,然后进行核苷酸/氨基酸相似性分析和系统发育分析。从696份标本中检出肠道病毒阳性标本412份,其中101份为CV-A16,76份为EV-A71,16份为CV-A4。9份CV-A4阳性标本成功病毒分离,并进行了全长VP1区核苷酸序列分析,结果表明,其核苷酸相似性为91.04%～100%,氨基酸相似性为95.24%～100%。系统进化分析显示9株CV-A4烟台株均属于G1基因群。与参考株CVA4-High Point-AY421762-genome相比,氨基酸序列的第20、34,102、111、143、184、185、200及304位氨基酸出现突变,本研究的9株分离株的这些位点也有不同,表明烟台地区的CV-A4有其独特的突变位点。CV-A4是引起2015年烟台地区手足口病发病的病原体之一,本次分离到的CV-A4均属于G1基因群进化分支。We wished to undertake etiologic surveillance of hand,foot and mouth disease（HFMD）and to analyze the molecular epidemiologic characteristics of coxsackievirus A4（CV-A4）.Real-time fluorescence reverse transcription-polymerase chain reaction of 696 HFMD samples in Yantai in 2015 revealed enterovirus（EV）,coxsackievirus A16（CV-A16）and intestinal virus A71（EV-A71）.Virus separation,determination of nucleotide/amino-acid sequences and phylogenetic analyses were done.We found 412 EVs from 696 specimens,101 of which were CV-A16,76 were EV-A71 and 16 were CV-A4.Nine CV-A4 positive specimens were isolated,and nucleotide-sequence analyses of the whole VP1 region were conducted.The nucleotide similarity was 91.04%-100%,and amino-acid similarity was 95.24%-100%.Compared with reference strains,20,34,102,111,143,184,185,200,304 amino-acid mutations were different.CV-A4 was found to be a branch of the G1 gene group.

关 键 词：手足口病(HFMD) 柯萨奇病毒A4(CV-A4) 系统发育 

分 类 号：R373.2[医药卫生—病原生物学]