RNA病毒表达调控研究中的RNA结构分析技术  

RNA Structural Probing Technologies Applied for Regulation of Expression of RNA Viruses

作　　者：王德亚 于成明[1] 丁诚实 原雪峰[1] WANG Deya;YU Chengming;DING Chengshi;YUAN Xuefeng(Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University Tai＇an 271018, China;College of Life Sciences, Zaozhuang University, Zaozhuang , 277160, China)

机构地区：[1]山东农业大学植物保护学院植物病理系,泰安271018 [2]枣庄学院生命科学学院,枣庄277160

出　　处：《病毒学报》2018年第4期579-585,共7页Chinese Journal of Virology

基　　金：国家自然科学基金面上项目(项目号:31670147),题目:烟草丛顶病毒RNA依赖的RNA聚合酶(RdRp)-1型移码翻译机制研究;国家自然科学基金面上项目(项目号:31370179),题目:烟草丛顶病毒不依赖帽子翻译机制的RNA结构基础解析~~

摘　　要：RNA病毒的多个生命过程如基因组复制、蛋白翻译等均需要特定RNA元件的调控,RNA结构是其发挥调控作用的分子基础,研究这些RNA元件结构及其在调控过程中的动态变化,将有助于深度解析相应过程的调控机制。RNA结构分析技术可以解析RNA元件的结构（二级结构和三级结构）。目前,RNA结构体分析技术主要有以下几种：Rnase酶法、In-line probing技术、SHAPE技术（Selective 2’-hydroxyl acylation analyzed by primer extension）、核磁共振技术、RNA结晶以及体内分析技术。本文将对RNA结构分析技术的发展历程、具体技术原理和操作细节、未来发展进行叙述,为相关科研工作提供技术参考。Cis-elements within the viral genome have essential roles in the genomic replication and protein translation of RNA viruses.The regulatory basis of cis-elements relies on the specific structure with which some viral or host factors can interact.Therefore,structural analyses of these cis-elements as well as their dynamic conformational shifts during processes can aid identification of the mechanisms involved in their regulation of different processes.Structural probing of RNA comprises analyses of secondary and tertiary structure.The methods described in this article include chemical/enzymatic modifications,in-line probing,selective 2′-hydroxyl acylation analyzed by primer extension,nuclear magnetic resonance,RNA crystallization,and in vivo structural probing.Moreover,the history,development,current hotspots and future direction of RNA structural probing are also summerized which may provide references for associated fields of research.

关 键 词：RNA病毒 表达调控 RNA结构 

分 类 号：S432.4[农业科学—植物病理学]