Toll样受体2在耐多药肺结核患者外周血中性粒细胞上的水平及意义  

The levels and significance of Toll like receptor 2 on peripheral blood polymorphonuclear cells of multidrug-resistant pulmonary tuberculosis

作　　者：刘婷[1] 赖惠婷[1] 郭夏娜[1] LIU Ting;LAI Huiting;GUO Xia＇na(Department of Clinical Laboratory,Bao＇an Hospial of Chronic Diseases Control and Prevention,Shenzhen, Guangdong 518100, China)

机构地区：[1]深圳市宝安区慢性病防治院检验科,广东深圳518100

出　　处：《中国热带医学》2018年第6期614-616,共3页China Tropical Medicine

基　　金：深圳市科技计划基础研究项目(No.JCYJ20160427092554740)

摘　　要：目的检测Toll样受体(TLR)2在耐多药肺结核患者外周血中性粒细胞(PMN)的水平,探讨其在结核分枝杆菌耐药机制中的意义。方法在药物干预前后,应用酶联免疫吸附试验(ELISA)技术,分别检测26例耐多药肺结核患者、30例非耐药肺结核患者和30例正常对照者外周血PMN培养上清液中TLR2的水平。结果干预前耐药组肺结核(PTB)患者外周血PMN培养上清液中TLR2的平均浓度为(11.789 6±3.346 0)ng/mL,低于非耐药组PTB患者外周血PMN培养上清液中TLR2的平均浓度(17.878 0±1.326 4)ng/mL(P=0.000)。同时,耐药组TLR2水平也低于正常对照组(14.719 7±2.635 4)ng/mL(P=0.001)。干预前后,耐药组TLR2水平差异无统计学意义,而非耐药组患者外周血PMN上TLR2的水平与干预前相比有降低(P=0.000)。结论 TLR2可能在耐多药结核分枝杆菌对宿主产生免疫逃避上发挥了关键作用,对于其具体作用机制,仍需进一步研究。Objective To detect the levels of TLR2 on peripheral blood polymorphonuclear cell （PMN） in patients with multidrug-resistant pulmonary tuberculosis （MDR-PTB） and explore their significance in the drug resistance mechanism of Mycobacterium tuberculosis. Methods Totally 56 patients with MTB were divided into two groups： MDR-MTB group （n=26） and non-MDR-MTB group （n=30） .Meanwhile, 30 healthy volunteers were assigned as control group.Followed by any treatments, ELISA assays were employed to detect TLR2 levels in the supernatant of peripheral blood PMN. After systemic treatments , TLR2 levels in MTB patients of two groups were detected again. Results Before treatment,the TLR2 average level of PMN culture supernatant in MDR-MTB group was （11.789 6±3.346 0） ng/mL, which was significantly lower than non-MDR-MTB group （17.878 0±1.326 4） ng/mL and control group （14.719 7±2.635 4）ng/mL,（P=0.000, P=0.001,respectively）. There was no statistic difference of TLR2 in MDR-MTB group pre- and post- the treatment.However, the TLR2 levels decreased significantly in non-MDR-MTB after treatment,compered with pre-treatment（P=0.000）. Conclusion These finding indicated that TLR2 may play the critical roles in immune evasion of MDR-MTB. However, more studies should be carried out to further evaluation.

关 键 词：耐多药结核病 中性粒细胞 TOLL样受体2 

分 类 号：R521[医药卫生—内科学]