阿米替林抗抑郁的免疫机制研究  

Study on the antidepressant immunologic mechanisms of Amitriptyline

作　　者：郭英 徐娅娟 姚晓静 杨佳妮 和润杰 白宇凡 莫正纪[2] 罗海芸 GUO Ying;XU Yajuan;YAO Xiaojing;YANG Jiani;HE Runjie;BAI Yufan;MO Zhengji;LUO Haiyun(School of Basic Medical Sciences, Kunming Medical University, Kunming, Yunnan, 650000 P. R. China;West China College of Pharmacy, Sichuan University, Chengdu , Sichuan , 610000 P. R. China)

机构地区：[1]昆明医科大学基础医学院,昆明云南650000 [2]四川大学华西药学院,成都四川610000

出　　处：《华西药学杂志》2018年第3期242-244,共3页West China Journal of Pharmaceutical Sciences

基　　金：云南省科技厅-昆明医科大学联合专项(2017FE467-160);昆明医科大学大学生创新性实验项目(2017~2018)

摘　　要：目的探讨阿米替林通过对小胶质细胞的免疫调节来发挥抗抑郁作用的机制。方法利用脂多糖（LPS）刺激小胶质细胞,采用Western blot检测磷酸化蛋白激酶B（p-AKT）、蛋白激酶B（AKT）、核因子-κB（NF-κB）、白细胞介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）及脑源性神经营养因子（BDNF）的蛋白表达;采用免疫荧光法检测IL-1β、TNF-α、BDNF的蛋白表达。结果阿米替林可降低被LPS激活的小胶质细胞中p-AKT/AKT、NF-κB、TNF-α、IL-1β的蛋白表达,增加BDNF的蛋白表达。结论阿米替林可能通过调控AKT/NF-κB信号通路,减少炎症因子TNF-α、IL-1β的释放,增加BDNF的蛋白表达,从而保护小胶质细胞,发挥抗抑郁作用。OBJECTIVE To investigate the antidepressant immunologic mechanism of Amitriptyline on microglia. METHODS The cell model with neuroinflammation were established by LPS stimulation. Expressions of p-AKT,AKT,NF-κB,IL-1β,TNF-α and BDNF protein in the microglia were determined by Western blot. IL-1β,TNF-α and BDNF protein in the microglia were observed by Immunofluorescence method. RESULTS The results showed that Amitriptyline pretreated with microglia decreased the expression of p-AKT/AKT,NF-ΚB,TNF-α,IL-1β protein and upregulated the protein expression of BDNF. CONCLUSION This study demonstrates that Amitriptyline may protect microglia through regulating AKT/NF-κB signal pathway,decreasing the release of TNF-α and IL-1β,and increasing the protein expression of BDNF,therefore,Amitriptyline plays a role in antidepressant.

关 键 词：阿米替林 小胶质细胞 蛋白激酶B 白细胞介素1Β 肿瘤坏死因子α 脑源性神经营养因子 抗抑郁 免疫机制 

分 类 号：R96[医药卫生—药理学]