分化抑制因子3在胚胎植入中的作用  

Role of inhibitors of differentiation 3 in embryo implantation

作　　者：叶亮 张雪[1] 王倩[1] 刘学庆[1] 张爽[1] 陈雪梅[1] 何俊琳[1] 高茹菲[1] 王应雄[1] 丁裕斌[1] Ye Liang;Zhang Xue;Wang Qian;Liu Xueqing;Zhang Shuang;Chert Xuemei;He Junlin;Gao Rufei;Wang Yingxiong;Ding Yubin(Laboratory of the Reproductive Biology, College of Public Health and Management, Chongqing Medical Universit;Joint International Research Laboratory of Reproduction ＆ Developmen)

机构地区：[1]重庆医科大学公共卫生与管理学院生殖生物学实验室教育部生殖与发育国际合作联合实验室,重庆400016

出　　处：《重庆医科大学学报》2018年第6期829-836,共8页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:81671493)

摘　　要：目的:检测分化抑制因子3(inhibitors of differentiation 3,Id3)基因在小鼠早期妊娠模型和人工诱导蜕膜化模型中子宫内膜中的表达规律;探究Id3基因在小鼠子宫内膜基质细胞蜕膜化过程的作用。方法:应用Western blot及q RT-PCR检测Id3在早期妊娠和人工诱导蜕膜化模型小鼠子宫中的表达;利用分离的原代小鼠子宫内膜基质细胞体外诱导蜕膜化、过表达Id3基因,分析Id3对基质细胞蜕膜化的影响。结果:Id3在小鼠早期妊娠模型中高表达于子宫内膜容受期形成阶段(孕第4天)(P蛋白质=0.001,Pm RNA=0.001),胚胎着床后基质细胞发生分化的蜕膜化子宫中表达较低(PD5蛋白质=0.026,PD5m RNA=0.038);体内人工诱导蜕膜化小鼠子宫内膜Id3的表达明显低于未发生蜕膜化的对照子宫(P蛋白质=0.005,Pm RNA=0.000);体外人工诱导蜕膜化可抑制Id3基因的表达(P蛋白质=0.000,Pm RNA=0.001);过表达Id3可以明显降低体外子宫内膜基质细胞蜕膜化过程;Stat3信号参与调控Id3的表达调控子宫内膜基质细胞蜕膜化过程。结论:Id3参与小鼠胚胎植入并调控子宫内膜基质细胞蜕膜化过程。Objective：To investigate the expression profile of inhibitors of differentiation 3（Id3） in the endometrium of early pregnant mice and artificially induced pregnant mice, and the role of Id3 gene in decidualization. Methods：Western blot and qRT-PCR were used to detect the expression of Id3 in uterus of early pregnancy and artificial induced decidualization model. The effect of Id3 expres- sion on the deeidualization of endometrial stromal cells was analyzed by the deeidualization and overexpression of Id3 gene induced by the isolated endometrial stromal cells of primary mice in vitr0. Results ： The Id3 highly expressed in the early pregnancy model of mice in the endometrial receptivity stage （the fourth day of pregnancy）（Pprotrin=0.001;PmRNA=0.001 ）, and lower expressed in decid- ualized uterus after embryonic implantation（PD5protrin=0.026;PD5mRNA=0.038）. The expression of Id3 in artificial induced endometrium was significantly lower than that in the control group（Pprotrin=0.005;PmRNA=0.000）. In vitro induced decidualization inhibited the expres- sion of Id3 gene （Pprotrin=0.000;PmRNA=0.001）. Overexpression of Id3 significantly reduced the process of decidualization of endometrial stromal cells in vitro;Stat3 signaling was involved in the process of decidual stromal cells regulated by Id3. Conclusion：Id3 is in- volved in the mice embryo implantation and regulates the decidualization of endometrial stromal cells.

关 键 词：子宫内膜 蜕膜化 分化抑制因子3 STAT3 

分 类 号：R321.3[医药卫生—人体解剖和组织胚胎学]