基于回归分析模型的复方脑脉通配伍规律研究  

Study on the Compatibility Regulation of Naomaitong Formula Based on Regression Analysis Model

作　　者：李恒 郭先帅 樊旭蕾 张雨 李克宁[1,2,3] 王淑美 Li Heng;Guo Xianshuai;Fan Xulei;Zhang Yu;Li Kening;Wang Shumei(Guangdong Pharmaceutical University, Guangzhou 510006;Research Center for Traditional Chinese Medicine Quality Engineering Technology Under Guangdong Universities, Guangzhou 510006;The Key Unit of Chinese Medicine Digitalization Quality Evaluation of State Administration of Traditional Chinese Medicine, Guangzhou 510006, China)

机构地区：[1]广东药科大学,广东广州510006 [2]广东省(高校)中药质量工程技术研究中心,广东广州510006 [3]国家中医药管理局中药数字化质量评价技术重点研究室,广东广州510006

出　　处：《广东化工》2018年第12期23-24,45,共3页Guangdong Chemical Industry

基　　金：国家自然科学基金(81274060;81473413)

摘　　要：目的:运用中药血清药理学,建立回归分析模型,初步探索复方脑脉通的配伍规律。方法:将动物随机分为空白对照组、复方脑脉通组、单味药组、配伍组,获取含药血清,分别给药四种细胞损伤模型组,MTT法测定细胞存活力,采用归一化法对数据进行处理分析。结果:初步确定复方中各药味的作用大小:大黄>人参、川芎>葛根,且葛根在给药6 h后对细胞保护作用随时间逐渐增大。结论:方中四味药起效贡献度大小不同,符合君臣佐使配伍规律,表明方剂配伍设计合理,同时为下一步PK-PD拟合研究复方脑脉通治疗脑中风的配伍规律打下基础。Objective： To initially explore the compatibility regulation of Naomaitong formula based on serum pharmacology of Chinese medicine by the establishment of regression analysis model. Methods： The animals were randomly divided into control group, Naomaitong formula group, 4 single herb groups, 10 compatibility groups, separated blood serum was prepared. Four kinds of cell injury model groups were given respectively, and cell viability was measured by MTT method. The data are processed and analyzed by normalization method. Results： The effects of each medicine in Naomaitong formula were preliminarily determined： rhubarb〉ginseng and chuanxiong rhizorna〉puerariae, and the protective effects of Pueraria on the cells gradually increased with time. Conclusion： Four herbs in Naomaitong Ibrmuta contributed to the effectiveness of different sizes, Satisfied with the rule of monarch Sasuke compatibility, showed that the compatibility of prescriptions was reasonable and laid the foundation for the PK-PD fitting study on the compatibility of Naomaitong formula in the treatment of stroke.

关 键 词：中药血清药理学 回归分析 脑脉通 配伍规律 

分 类 号：R289.1[医药卫生—方剂学]