双罗丹明类衍生物荧光探针的合成及对Cu^(2+)的识别  

Synthesis of a Birhodamine-based Fluorescent Probe and Recognition Properties for Cu^(2+)

作　　者：袁跃华[1] 朱永军[1] 殷强锋[2] 冯锋[1] 王海雁[1] 田茂忠[1] YUAN Yue-hua;ZHU Yong-jun;YIN Qiang-feng;FENG Feng;WANG Hai-yan;TIAN Mao-zhong(College of Chemistry and Environmental Engineering, Shanxi Datong University, Datong 037009;Chemical Engineering Institute ,East China University of Science and Technology ,Shanghai 200237)

机构地区：[1]山西大同大学化学与环境工程学院,山西大同037009 [2]华东理工大学化工学院,上海200237

出　　处：《分析科学学报》2018年第3期327-331,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21175085;21375083);大同市基础研究计划项目(No.2017126)

摘　　要：设计并合成了一种可检测Cu^(2+)的新双罗丹明类衍生物荧光增强型分子探针(RG1),并用核磁和高分辨质谱(HRMS)对其结构进行了表征。通过紫外-可见光谱和荧光光谱研究了该荧光分子探针对Cu^(2+)的识别性能,结果表明:在体积比为1∶1的乙腈-水(10mmol/L Tris-HCl,pH=7.2)溶液体系中,探针RG1本身无色且荧光很弱,加入Cu^(2+)后,探针在553nm处出现强的荧光发射峰,溶液颜色从无色变为粉红色,对Cu^(2+)表现出好的选择性。此外,该探针可在较宽的pH范围(5~10)内直接检测Cu^(2+)的浓度。结果表明,Cu^(2+)浓度在3.0×10^(-6)~1.5×10^(-5)mol/L范围内与探针的荧光强度呈良好的线性关系,Cu^(2+)检出限为3.31×10^(-7)mol/L。同时对河水进行加标回收实验,得到了良好的回收率。A novel birhodamine-based fluorescent enhancement molecular probe（RG1）for the detection of Cu^2＋ was designed and synthesized.Its structure was characterized by ^1H NMR,^13C NMR and high resolution mass spectrometer（HRMS）.The recognition properties of this fluorescent molecular probe for Cu^2＋were studied by UV-Vis spectra and fluorescence spectra.The results showed that in acetonitrile-water（1∶1,V/V）solution the probe was colorless and the fluorescence intensity was very weak.Upon the addition of Cu^2＋,a new fluorescence emission peak at 553 nm appeared with increasing intensity evidently.The color of the solution changed from colorless to pink.Probe RG1 showed good selectivity to Cu^2＋.In addition,the probe could detect the concentration of Cu^2＋ directly in a wide range of pH（5-10）.The concentration of Cu^2＋ was in good linearity with the fluorescence intensity of the probe RG1 in the range of 3.0×10^-6-1.5×10^-5mol/L with a detection limit of 3.31×10^-7mol/L.At the same time,the standard recovery experiments of river sample were carried out with satisfactory result.

关 键 词：荧光探针 罗丹明6G 铜离子 

分 类 号：O657.39[理学—分析化学]