基质细胞衍生因子-1和趋化因子受体-4在子宫内膜异位症患者病灶中的表达及意义  

The expressions and the roles of SDF-1/CXCR-4 and SDF-1/CXCR-4 in human endometriosis

作　　者：欧阳卓[1] 孙晋萍[1] 田秀兰[1] 陈敏秀[1] 翟建军[1] Ouyang Zhuo;Sun Jinping;Tian Xiulan;Chen Minxiu;Zhai Jianjur(Department of Gynecology and Obstetrics, Beijing Tongren Hospital, Capital Medical University ,Beijing 100730, China)

机构地区：[1]首都医科大学附属北京同仁医院妇产科,100730

出　　处：《中华医学杂志》2018年第23期1854-1858,共5页National Medical Journal of China

摘　　要：目的探讨基质细胞衍生因子-1（SDF-1）和趋化因子受体-4（CXCR-4）在子宫内膜异位症（EM）中的表达及意义。方法选取2016年1月1日至2017年12月31日在首都医科大学附属北京同仁医院手术治疗的50例子宫肌瘤患者为对照组即非EM组，50例EM患者为试验组。经手术收集子宫肌瘤患者的子宫内膜组织、卵巢子宫内膜异位病灶组织和腹腔液标本。采用RT-PCR法、ELISA法、免疫组化法检测SDF-1和CXCR-4在二组的表达情况。采用CCK-8法检测SDF-1和CXCR-4在子宫内膜异位间质细胞分裂增殖中的作用。结果SDF-l和CXCR4在EM患者腹水或腹腔液中的浓度的平均值分别是（2．56±0．33）mg／L和（4．47±0．32）mg／L。在子宫肌瘤患者腹水或腹腔液中浓度的平均值分别是（1．39±0．36）mg／L和（3．16±0．32）mg／L。SDF-1和CXCR-4在子宫内膜异位症患者腹水或腹腔液中的表达均明显高于非EM组（P〈0．05）。SDF．1和CXCR-4在EM病灶和正常子宫内膜组织中的腺上皮细胞和问质细胞中均有表达，免疫染色阳性部位均定位于细胞质。免疫染色的表达量采用吸光度值来计算分析及比较。EM组SDF-1和CXCR-4的平均值分别是0．21±0．13和0．21±0．13，正常子宫内膜组织中的平均值分别是0．15±0．13和0．14±0．13。两者在EM组织中的表达均明显高于正常子宫内膜组织，差异均有统计学意义（均P〈0．05）。体外分离培养的EM问质细胞中有丰富的CXCR4mRNA的表达。SDF-1呈剂量依赖性促进体外培养的子宫内膜异位间质细胞的分裂增殖。其作用可使CXCR-4的中和抗体明显抑制。结论SDF-1和CXCR-4在EM中的高表达及SDF-1通过其特异性受体CXCR-4促进了子宫内膜异位问质细胞的分裂增殖，提示，两者在EM的发病机制中扮演了重要的角色。Objective To investigate the expressions and significances of SDF-1 and its receptor CXCR-4 in endometriosis. Methods 50 hysteromyoma patients treated at Beijing Tongren Hospital, Capital Medical University between 1 st January 2016 and 31st December 2017 were divided into control group, that is, non-endometriosis group, while another 50 endometriosis patients were divided into experimental group. The endometrial tissues, endometriosis lesions, and peritoneal fluid samples of hysteromyoma patients were collected by operation. RT-PCR, ELISA and immunohistochemistry were adopted to detect the expressions of SDF-1 and CXCR-4 in the two groups. Cell count was used to analyze the roles of SDF-1 and CXCR4 in the mitosis and proliferation of endometrial stromal cells. Results The mean value of SDF-1 and CXCR-4 expressions in aseites or peritoneal fluid of endometriosis patients were （ 2. 56 ± 0. 33 ） mg/L and （4.47 ± 0. 32） rag/L, respectively. The mean concentrations in ascites or peritoneal fluid in hysteromyoma patients were （1.39 ±0. 36） mg/L and （3.16 ±0. 32） mg/L, respectively. The expressions of SDF-1 and CXCR-4 in ascites or peritoneal fluid of endometriosis patients were both significantly higher than those of patients in non-endometriosis group （P 〈 0. 05）. SDF-1 and CXCR-4 were expressed in both endometriosis lesions and the glandular epithelial cells and mesenehymal cells of normal endometrial tissue. Positive staining sites were located in the cytoplasm. A value was used to calculate and analyze the expression of immune staining. The mean A value of SDF-1 and CXCR-4 in endometriosis group were 0. 21 ± 0. 13 and 0.21 ±0. 13, respectively, and the mean A value in normal endometrial tissues were 0.15±0.13 and 0. 14±0. 13, respectively. The expressions of these two in endometrial tissues were significantly higher than that in normal endometrial tissues, and the differences were statistically significant （P 〈 0.05 ）. The expression of CXCR-4 mRNA was abundant in the mesenchyma

关 键 词：SDF-1 CXCR-4 子宫内膜异位症 

分 类 号：R711.71[医药卫生—妇产科学]