山杏仁蛋白酶水解物抑制炎症因子NO、TNF-α和IL-1β的产生  

Production Inhibition of Inflammatory Factors NO,TNF-α and IL-1β by Apricot Kernel Protein Hydrolysate

作　　者：王萌 王晨[1,2] 于哲 刘湘婷[1,2] 焦慧俊 方顺翔[1,2] 许美玉 WANG Meng;WANG Chen;YU Zhe;LIU Xiang- ring;JIAO Hui-jun;FANG Shun-xiang;XU Mei-yu(Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China;Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University,Beijing 100083 ,China)

机构地区：[1]北京林业大学,生物科学与技术学院食品科学与工程系,北京100083 [2]北京林业大学,林业食品加工与安全北京市重点实验室,北京100083

出　　处：《食品工业科技》2018年第13期296-300,336,共6页Science and Technology of Food Industry

基　　金：北京市级大学生创新创业计划(S201610022052).

摘　　要：分别用碱性蛋白酶、中性蛋白酶、木瓜蛋白酶、胃蛋白酶和胰蛋白酶制备山杏仁蛋白酶水解物,通过格里斯实验和ELISA方法,研究山杏仁蛋白酶水解物对巨噬细胞产生炎症因子NO、TNF-α和IL-1β的影响。结果表明山杏仁木瓜蛋白酶水解物显著抑制炎症因子NO、TNF-α和IL-1β产生,其抑制率分别为62%、11%和21%,而其它四种蛋白酶水解物没有显示明显的抗炎作用。将山杏仁木瓜蛋白酶水解物分离制备为5~10,3~5,1~3 k Da和小于1 k Da等不同分子量组分,进一步研究其抗炎活性,结果表明小于1 k Da低分子量组分具有较高抗炎活性,其对NO、TNF-α和IL-1β产生的抑制率分别为81%、59%和46%。分析氨基酸组成与含量结果表明,抗炎活性较强的小于1 k Da组分中,疏水性氨基酸和亲水性氨基酸含量均较高,同时谷氨酸、天冬氨酸和精氨酸等具有抗炎作用的氨基酸含量也较高。以上结果表明,山杏仁蛋白被木瓜蛋白酶水解后能够释放抗炎生物活性肽,该活性肽的分子量小于1 k Da,可能是具有两亲性的结构。Apricot kernel protein was hydrolyzed by alkaline protease, neutral protease, papain, pepsin and trypsin, respectively.The effects of apricot protein enzymatic hydrolysate on production of inflammatory factors such as NO,TNF-α and IL-1β produced by macrophage cell were investigated by Griess assay and ELISA. The results showed that the papain hydrolysate could inhibit the production of NO,TNF-α and IL-1β, the inhibition rates were 62% , 11% ,21% , while the other four enzyme hydrolysates did not show significant anti-inflammatory effects.Apricot kernel protein enzymatic hydrolysates was separated by ultrafiltration into four fractions based on molecular weight, 5 - 10,3 - 5,1 - 3 kDa and below 1 kDa, and investigated their anti-inflammatory effects.The results showed that the inhibitory rates of the lower molecular weight fractions （below 1 kDa）on NO,TNF-α and IL-1β were 81% ,59% ,46% , respectlvely.Results of amino acid composition and content of different peptides assay indicated that the fraction of lower than 1 kDa with stronger anti-inflammatory activity were not only rich in hydrophilic amino acids, but also had abundant hydrophobic amino acids.At the same time, the anti-inflammatory amino acids content in the fraction were also higher, such as glutamic acid, aspartic acid and arginine.These results indicated that anti -inflammatory bioactive peptides could be released from apricot kernel protein through hydrolyzing by papain, which were might be peptides with below 1 kDa molecular weight and an amphiphilic structure.

关 键 词：山杏仁蛋白酶水解物 抗炎 一氧化氮 肿瘤坏死因子Α 白细胞介素-1Β 

分 类 号：TS201.4[轻工技术与工程—食品科学]