建立测定槲皮素及其甲基化产物的高效液相色谱-质谱法  

The Simultaneous Determination of Quercetin and Its Methylated Metabolites by High Performance Liquid Chromatography-Mass Spectrometry

作　　者：李素云[1,2] 吉琳琳 高蔚娜[2] 郭长江 李峥 张振清 

机构地区：[1]首都医科大学附属北京世纪坛医院,100038 [2]军事科学院军事医学研究院环境医学与作业医学研究所,300050 [3]军事科学院军事医学研究院毒物药物研究所,100850

出　　处：《首都食品与医药》2018年第12期44-46,共3页Capital Food Medicine

基　　金：国家自然科学基金面上项目(第81372988)

摘　　要：目的建立同时测定同一体系中槲皮素、异鼠李亭和柽柳黄素的高效液相色谱-质谱(HPLC-MS)检测方法。方法将槲皮素、异鼠李亭和柽柳黄素溶解于磷酸缓冲液(PBS)中,以丁螺环酮为内标,进行HPLC-MS测定。色谱柱选择Agilent-C_(18)柱,流动相为甲醇:乙腈(1∶1),等梯度洗脱,离子源为ESI(+),采用选择离子监测(SIM)模式检测。结果槲皮素、异鼠李亭、柽柳黄素在1~1000μg/L范围内线性关系良好,相关系数分别为0.99996、0.99955、0.99983;方法的准确度和精密度良好。结论该方法操作简便、灵敏度高、准确度好,可应用于槲皮素及其甲基化代谢产物异鼠李亭和柽柳黄素的测定。Objective Tried to create a high performance liquid chromatographymass spectrometry(HPLC-MS) method to determination of quercetin, isorhamnetin and tamarixetin.Methods Quercetin,isorhamnetin and tamarixetin were determined simultaneously by HPLC-MS,with buspirone as the internal standard.The samples were divided by an Agilent-C18 and then analyzed in positive ion electrospray mode.The mobile phase including methanol and acetonitrile(1:1).Results The calibration curve for quercetin,isorhamnetin and tamarixetin ranging from 1 to 1000μg/L,with correlation coefficients of 0.99996,0.99955 and 0.99983 respectively.The accuracy and precision both met the requirement of the experiment.Conclusion The method is sensitive, precise,and can quickly determinate quercetin,isorhamnetin and tamarixetin simultaneouslyin phosphate buffer solution(PBS).

关 键 词：槲皮素 异鼠李亭 柽柳黄素 高效液相色谱-质谱法 

分 类 号：R284.1[医药卫生—中药学]