Evolutionary analysis of plant jacalin-related lectins(JRLs)family and expression of rice JRLs in response toMagnaporthe oryzae  

作　　者：HAN Yi-juan ZHONG Zhen-hui SONG Lin-lin Olsson Stefan WANG Zong-hua LU Guo-dong 

机构地区：[1]State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops/Key Laboratory of Biopesticide and Chemistry Biology,Ministry of Education/College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China

出　　处：《Journal of Integrative Agriculture》2018年第6期1252-1266,共15页农业科学学报（英文版）

基　　金：funded by the National Key Research and Development Program of China(2016YFD0100600);the National Natural Science Foundation of China(U1405212)

摘　　要：Jacalin-related lectins （JRLs） are widely distributed carbohydrate-binding proteins in the plant kingdom, which play key roles in development and pathogen defense. In this study, we profiled evolutionary trajectory of JRLs family in 30 plant species and identified domain diversification and recombination leading to different responsive patterns of JRLs in rice during defense against rice blast. All of 30 plant species analyzed in our study have two types of JRLs by containing either a single jacalin or repeated jacalin domains, while chimeric jacalins exist in more than half of the species, especially in the Poaceae family. Moreover, Poaceae species have evolved two types of unique chimeric JRLs by fusing the jacalin domain（s） with dirigent or NB_ARC domain, some of which positively regulate plant immunity. Seven Poaceae-specific JRLs are found in the rice genome. We further found expression of rice JRLs, including four Poaceae-specific JRLs, are induced by Magnaporthe oryzae infections at either early or late infection stages. Overall, the results present the evolutionary trajectory of JRLs in plant and highlight essential roles of Poaceae specific JRLs against pathogen attacks in rice.Jacalin-related lectins （JRLs） are widely distributed carbohydrate-binding proteins in the plant kingdom, which play key roles in development and pathogen defense. In this study, we profiled evolutionary trajectory of JRLs family in 30 plant species and identified domain diversification and recombination leading to different responsive patterns of JRLs in rice during defense against rice blast. All of 30 plant species analyzed in our study have two types of JRLs by containing either a single jacalin or repeated jacalin domains, while chimeric jacalins exist in more than half of the species, especially in the Poaceae family. Moreover, Poaceae species have evolved two types of unique chimeric JRLs by fusing the jacalin domain（s） with dirigent or NB_ARC domain, some of which positively regulate plant immunity. Seven Poaceae-specific JRLs are found in the rice genome. We further found expression of rice JRLs, including four Poaceae-specific JRLs, are induced by Magnaporthe oryzae infections at either early or late infection stages. Overall, the results present the evolutionary trajectory of JRLs in plant and highlight essential roles of Poaceae specific JRLs against pathogen attacks in rice.

关 键 词：jacalin-related lectins phylogeny Oryza sativa Magnaporthe oryzae infection responses 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]