A study on the pathogen species and physiological races of tomato Fusarium wilt in Shanxi,China  

作　　者：CHANG Yin-dong DU Bin WANG Ling JI Pei XIE Yu-jie LI Xin-feng LI Zhi-gang WANG Jian-ming 

机构地区：[1]College of Agriculture,Shanxi Agricultural University [2]Department of Horticulture,Taiyuan University [3]Technology Center Yuncheng Sub-Center,Shanxi Entry-Exit Inspection and Quarantine Bureau [4]Department of Genetics and Biochemistry,Clemson University

出　　处：《Journal of Integrative Agriculture》2018年第6期1380-1390,共11页农业科学学报（英文版）

基　　金：partially supported by the Shanxi Provincial Science and Technology Planning Project,China (20120311019-3);the Shanxi Provincial Science and Technology Foundation Platform Construction Project,China (1105-0104);the Shanxi Provincial Graduate Education Innovation Project,China (2017BY065)

摘　　要：In order to clarify the main pathogens of tomato Fusarium wilt in Shanxi Province, China, morphological identification, elongation factor 1 alpha （EF-1α） sequence analysis, specific primer amplification and pathogenicity tests were applied to study the isolates which were recovered from diseased plants collected from 17 different districts of Shanxi Province. The results were as follows： 1） Through morphological and molecular identification, the following 7 species of Fusarium were identified： F. oxysporum, F. solani, F. verticillioides, F. subglutinans, F. chlamydosporum, F. sporotrichioides, and F. semitectum; 2） 56 isolates of F. oxysporum were identified using specific primer amplification, among which, 29, 5 and 6 isolates were respectively identified as F. oxysporum f. sp. lycopersici physiological race 1, race 2, and race 3; 3） pathogenicity test indicated the significant pathogenicity of F. oxysporum, F. solani, F. verticillioides, and F. subglutinans to tomato plant. Therefore, among these 4 species confirmed as pathogenic to tomato in Shanxi, the highest isolation rate （53.3％） corresponded to F. oxysporum. Three physiological species, race 1, race 2, and race 3 of F. oxysporum f. sp. lycopersici are detected in Shanxi, among which race 1 is the most widespread pathogen and is also considered as the predominant race.In order to clarify the main pathogens of tomato Fusarium wilt in Shanxi Province, China, morphological identification, elongation factor 1 alpha （EF-1α） sequence analysis, specific primer amplification and pathogenicity tests were applied to study the isolates which were recovered from diseased plants collected from 17 different districts of Shanxi Province. The results were as follows： 1） Through morphological and molecular identification, the following 7 species of Fusarium were identified： F. oxysporum, F. solani, F. verticillioides, F. subglutinans, F. chlamydosporum, F. sporotrichioides, and F. semitectum; 2） 56 isolates of F. oxysporum were identified using specific primer amplification, among which, 29, 5 and 6 isolates were respectively identified as F. oxysporum f. sp. lycopersici physiological race 1, race 2, and race 3; 3） pathogenicity test indicated the significant pathogenicity of F. oxysporum, F. solani, F. verticillioides, and F. subglutinans to tomato plant. Therefore, among these 4 species confirmed as pathogenic to tomato in Shanxi, the highest isolation rate （53.3％） corresponded to F. oxysporum. Three physiological species, race 1, race 2, and race 3 of F. oxysporum f. sp. lycopersici are detected in Shanxi, among which race 1 is the most widespread pathogen and is also considered as the predominant race.

关 键 词：tomato Fusarium wilt Fusarium oxysporum f. sp. lycopersici physiological races EF-1α sequence analysis specific primer amplification 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]