Carbendazim sensitivity in populations of Colletotrichum gloeosporioides complex infecting strawberry and yams in Hubei Province of China  

作　　者：HAN Yong-chao ZENG Xiang-guo XlANG Fa-yun ZHANG Qing-hua GUO Cong CHEN Feng-ying GU Yu-chen 

机构地区：[1]Institute of Industrial Crops,Hubei Academy of Agricultural Sciences,Wuhan 430064,P.R.China [2]Hubei Key Laboratory of Crop Diseases,Insect Pests and Corp Weeds Control/Key Laboratory of Integrated Pest Management on Crops in Central China,Wuhan 430064,P.R.China

出　　处：《Journal of Integrative Agriculture》2018年第6期1391-1400,共10页农业科学学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(31701882);the Competitive Nature Project of the Hubei Academy of Agricultural Sciences,China(2016JZXJH006);the Agricultural Science and Technology Innovation Center Program of Hubei Province,China(2016-620-000-001-014)

摘　　要：The ascomycete fungus Colletotrichum gloeosporioides is a devastating plant pathogen with a wide host range and worldwide distribution. Carbendazim has been widely used to control anthracnose caused by the C. gloeosporioides complex in China for more than 30 years and resistance to carbendazim has been reported in China. A total of 125 Colletotrichum isolates of strawberry and yam were collected from different geographical regions in Hubei Province, China. Approximately 52.8％ of Colletotrichum spp. isolates showed resistance to carbendazim. The isolates tested in this study belong to four species, and the frequencies of resistant isolates differed across Colletotrichum species. Resistant isolates were found in C. siamense and C. fructicola. In contrast, all isolates of C. gloeosporioides and C. aenigma were sensitive to carbendazim. Highly carbendazim-resistant isolates harbored the E198A mutation in the β-tubulin 2 （TUB2） gene, whereas moderately carbendazim-resistant isolates harbored the F200Y mutation in the TUB2 gene. Carbendazim-sensitive Colletotrichum isolates in this study were not genetically similar enough to form a separate cluster from resistant isolates. The result of this study emphasizes the importance of knowing which Colletotrichum sp. is present, when strategies for disease control are made.The ascomycete fungus Colletotrichum gloeosporioides is a devastating plant pathogen with a wide host range and worldwide distribution. Carbendazim has been widely used to control anthracnose caused by the C. gloeosporioides complex in China for more than 30 years and resistance to carbendazim has been reported in China. A total of 125 Colletotrichum isolates of strawberry and yam were collected from different geographical regions in Hubei Province, China. Approximately 52.8％ of Colletotrichum spp. isolates showed resistance to carbendazim. The isolates tested in this study belong to four species, and the frequencies of resistant isolates differed across Colletotrichum species. Resistant isolates were found in C. siamense and C. fructicola. In contrast, all isolates of C. gloeosporioides and C. aenigma were sensitive to carbendazim. Highly carbendazim-resistant isolates harbored the E198A mutation in the β-tubulin 2 （TUB2） gene, whereas moderately carbendazim-resistant isolates harbored the F200Y mutation in the TUB2 gene. Carbendazim-sensitive Colletotrichum isolates in this study were not genetically similar enough to form a separate cluster from resistant isolates. The result of this study emphasizes the importance of knowing which Colletotrichum sp. is present, when strategies for disease control are made.

关 键 词：CARBENDAZIM resistance frequency Colletotrichum gloeosporioides Colletotrichum species point mutations ANTHRACNOSE 

分 类 号：S436.32[农业科学—农业昆虫与害虫防治] S436.68[农业科学—植物保护]