槐果碱对CCI致神经病理性疼痛小鼠氧化应激水平及Caspase-3/Bax/Bcl-2信号通路的影响  

Effect of sophocarpine on oxidative stress and Caspase-3/Bax/Bcl-2 signaling pathway in mice with neuropathic pain induced by chronic constriction injury

作　　者：金少举[1,2] 王蓉 李海龙[4] 任丽平 孙明振[1] 王雁梅[1] 王瑞[5] Jin Shaoju;Wang Rong;Li Hailong;Ren Liping;Sun Mingzhen;Wang Yanmei;Xu Songtao(Dept of pharmacology, Luohe Medical College, Luohe 462002;Tumor Occurrence and Prevention research innovation team of Henan, Luohe 462002;Dept of Nuclear Medicine, General Hospital of Ningxia Medical University, Yinchuan 750004;Dept of Respiratory Medicine, The Third People＇s Hospital of Ningxia, Yinchuan 750021;Dept of Pharmacy, The First Affiliated Hospital of Luohe Medical College, Luohe 462002)

机构地区：[1]漯河医学高等专科学校药理学教研室,漯河462002 [2]河南省肿瘤发生与防治研究创新型科技团队,漯河462002 [3]宁夏医科大学总医院核医学科,银川750004 [4]宁夏回族自治区第三人民医院呼吸内科,银川750021 [5]漯河医学高等专科学校第一附属医院药学部,漯河462002

出　　处：《中药药理与临床》2018年第2期39-43,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河南省基础与前沿技术研究计划项目;项目编号:152300410178

摘　　要：目的:探讨槐果碱缓解坐骨神经慢性缩窄性损伤(Chronic constriction injury,CCI)致神经病理性疼痛的抗氧化应激和抗凋亡机制。方法:将BALB/c小鼠随机分为:假手术组(sham)、神经病理性疼痛模型组(CCI)、普瑞巴林阳性药对照组(CCI+Pre10 mg/kg)及槐果碱组(CCI+SC 40、20、10 mg/kg),共6组,每组10只小鼠。采用CCI法建立小鼠神经病理性疼痛动物模型,于术前d1、术后d7(给药前d1)及给药后1、3、5、7 d测定小鼠热刺激缩足反应潜伏期及冷缩足反射次数;采用生化法测定槐果碱对CCI小鼠脊髓组织SOD、GSH-Px、T-AOC及MDA水平的影响;采用RT-PCR和Western blot法检测槐果碱对CCI小鼠脊髓组织Caspase-3、Bax和Bcl-2 mRNA及蛋白表达的影响。结果:与假手术组比较,CCI模型组小鼠术后PWTL显著缩短,CWT明显增多;小鼠脊髓组织SOD、GSH-Px、T-AOC水平降低、MDA含量增多、Caspase-3、Bax mRNA及蛋白表达上调、Bcl-2 mRNA及蛋白表达下调;与CCI模型组比较,槐果碱40 mg/kg组小鼠PWTL延长,CWT减少;小鼠脊髓组织SOD、GSH-Px、T-AOC水平升高、MDA含量减少、Caspase-3、Bax mRNA及蛋白表达下调、Bcl-2 mRNA及蛋白表达上调。结论:槐果碱对CCI致小鼠神经病理性疼痛具有较好的缓解作用,其机制可能与其抗过氧化应激和抗神经元凋亡有关。Objective： To explore the antioxidant stress and anti-apoptosis mechanism of sophocarpine（ SC） on mice with neuropathic pain induced by chronic constriction injury（ CCI）. Methods： BALB/c mice were randomly divided into six groups： the sham group,CCI model group,CCI ＋ Pregabalin（ Pre） 10 mg/kg group,and CCI ＋ SC（ 40 mg/kg,20 mg/kg and 10 mg/kg） groups,10 mice in each group. The animal model of neuropathic pain in mice was induced by CCI. The paw withdrawal thermal latency（ PWTL） and cold withdrawal times（ CWT）were detected to evaluate analgesic effects of SC in mice with neuropathic pain induced by CCI. Contents of SOD,GSH-Px,T-AOC and MDA in spinal cord tissues of mice were detected by biochemical methods. Expression levels of Caspase-3,Bax,and Bcl-2 mRNA and protein in spinal cord tissues of mice were detected by reverse transcription-polymerase chain reaction（ RT-PCR） and Western blot methods. Results：Compared with the sham group,mice’s PWTL in CCI model group shorten,CWT increased. Activities of SOD,GSH-Px,and T-AOC in spinal cord tissues were reduced,and the content of MDA was increased. Expression levels of Caspase-3 and Bax mRNA and protein in spinal cord tissues were up-regulated,and Bcl-2 down-regulated. Compared with CCI model group,mice’s PWTL in SC 40 mg/kg group was prolonged,CWT was decreased. Activities of SOD,GSH-Px,and T-AOC in spinal cord tissues were enhanced,and the content of MDA was decreased. Expression levels of Caspase-3 and Bax mRNA and protein in spinal cord tissues were down-regulated,and Bcl-2 up-regulated.Conclusion： SC could relieve mice’s neuropathic pain induced by CCI,and its mechanism was related to antioxidant stress and anti-apoptosis.

关 键 词：槐果碱 神经病理性疼痛 氧化应激 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]