八味痛风康微丸抗痛风作用的实验研究  

Experimental study on the anti gout effect of Bawei Tongfeng Kangwei Pills

作　　者：王丽苹 江涛 陈强威 庄素琪 温玉莹 唐春萍 Wang Liping;Jiang Tao;Chen Qiangwei;Zhuang Suqi;Wen Yuying;Tang Chunping(School of Traditional Chinese Medicine, Guangdong Pharmaceutical University;Laboratory Animal Center, Guangdong Pharmaceutical University, Guangdong 510006)

机构地区：[1]广东药科大学中药学院 [2]广东药科大学实验动物中心,广州510006

出　　处：《中药药理与临床》2018年第2期93-97,共5页Pharmacology and Clinics of Chinese Materia Medica

摘　　要：目的:研究八味痛风康微丸的抗痛风作用。方法:采用尿酸盐结晶(MSU)致大鼠急性痛风性关节炎模型及次黄嘌呤所致的小鼠高尿酸血症模型,观察八味痛风康微丸的抗痛风作用,并通过热板法、醋酸扭体法和甲醛法观察该药的镇痛作用;利用二甲苯致小鼠耳廓肿胀模型、角叉菜胶致大鼠足肿胀模型、小鼠毛细血管通透性模型,观察该药的抗炎作用。结果:八味痛风康微丸16.4g/kg以上剂量可明显抑制尿酸盐结晶(MSU)诱导的大鼠足跖肿胀,降低血清IL-1β和TNF-α水平,并能降低次黄嘌呤所致高尿酸血症小鼠的血尿酸水平;能提高小鼠的痛阈值,减少醋酸所致小鼠的扭体次数,并能够降低甲醛致小鼠镇痛实验中足中PGE2含量;八味痛风康微丸也可抑制醋酸所致小鼠毛细血管通透性的增加,抑制角叉菜胶大鼠足趾肿胀和二甲苯致小鼠耳廓肿胀。结论:八味痛风康微丸具有抗痛风、镇痛、抗炎作用,其作用机制可能与降低炎症因子IL-1β、、TNF-α和PGE2水平有关。Objective： To study the anti-gout effect of Bawei Tongfeng Kang Pills（ BTKP）. Methods： Acute gouty arthritis model induced by urate crystals（ MSU） and hyperuricemia model induced by hypoxanthine were used to observe the anti-gout effect of BTKP. The analgesic effect of the drug was observed by hot plate method,acetic acid writhing method and formaldehyde method. Xylene-induced mouse auricle swelling model,carrageenan-induced rat paw swelling model and mouse capillary permeability model were established in order to observe the anti-inflammatory effect of the drug. Results： BTKP significantly inhibited the paw edema induced by urate crystallization（ MSU）,decreased serum levels of IL-1β and TNF-α,and decreased serum uric acid levels by hypoxanthine-induced hyperuricemia in mice. It increased the pain threshold in mice,reduced the number of writhing induced by acetic acid in mice,and reduced foot PGE2 content in the analgesia model by formaldehyde in mice. BTKP also decreased the acetic acid-induced capillary permeability,xylene induced mouse ear swelling in mice and carrageenan rat toe swelling in rats. Conclusions： BTKP has anti-gout,analgesic and anti-inflammatory effects. Its mechanism may be related to decreasing levels of inflammatory cytokines IL-1β,TNF-α and PGE2.

关 键 词：八味痛风康微丸 痛风性关节炎 高尿酸血症 抗炎 镇痛 炎症因子 

分 类 号：R285.5[医药卫生—中药学]