中间锦鸡儿CiMYB185基因克隆及表达分析  

Cloning and Expression Analysis of CiMYB185 Gene from Caragana intermedia

作　　者：牛肖翠 郝志霞 雷凤燕 王光霞[1] 王瑞刚[1] Niu Xiaocui;Hao Zhixia;Lei Fengyan;Wang Guangxia;Wang Ruigang(College of Life Sciences, Inner Mongolia Agricultural University, Hohhot, 010018;Tongliao Quality ＆ Safety Centre of Agriculture and Livestock, Tongliao, 028000)

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018 [2]内蒙古通辽市农畜产品质量安全中心,通辽028000

出　　处：《分子植物育种》2018年第11期3518-3525,共8页Molecular Plant Breeding

基　　金：内蒙古自然科学基金(2016MS0340)资助

摘　　要：为进一步了解植物抗逆境胁迫的机理,本研究以中间锦鸡儿为试验材料,从实验室已建立的转录组数据库中选取转录因子CiMYB185编码基因作为研究对象。利用PCR技术分别以cDNA与g DNA为模板对CiMYB185基因进行全长克隆。测序结果表明:CiMYB185基因的开放阅读框为909 bp,编码303个氨基酸,其基因组DNA序列长度为1 592 bp,含有1个内含子和2个外显子。序列分析发现该基因所编码蛋白的N端包含2个MYB结构域,属于典型的R2R3-MYB类蛋白;利用染色体步移技术克隆得到908 bp的ATG上游启动子序列,序列中包含一些与光反应和激素相关的元件。该研究为进一步了解植物抗逆境胁迫的机理奠定了基础。To further understand the mechanism of plant resistance to stress, Caragana intermedia was selected as the material in this study, and the transcription factor encoding gene CiMYB185 from the transcriptional database in the laboratory was chosen as the research object. The full length of CiMYB185 gene was cloned by PCR technique, using cDNA and g DNA as the template. Sequencing results indicated that CiMYB185 had an open reading frame of 909 bp, encoding 303 amino acids protein. The full-length g DNA of CiMYB185 was 1 592 bp,including one introns and two exons. Sequence analysis showed that the N-terminal of the protein encoded by CiMYB185 contained two MYB domains, belonging to typical R2 R3-MYB protein. 908 bp ATG upstream promoter sequence was cloned by genome walking, which contained some elements associated with light reactions and hormones. This study laid the foundation for further understanding the mechanism of plant resistance to stress.

关 键 词：中间锦鸡儿 CiMYB185 启动子克隆 

分 类 号：Q943.2[生物学—植物学]