基于转录组测序数据的甘薯SSR标记开发及群体聚类分析  

SSR Markers Development and Group Cluster Analysis of Sweetpotato Based on Transcriptional Sequencing Data

作　　者：杨育峰[1] 史典义 王雁楠[1] 杨国红[1] 徐心志[1] 翟红[2] 何绍贞[2] 刘庆昌[2] 苏文瑾[3] Yang Yufeng;Shi Dianyi;Wang Yannan;Yang Guohong;Xu Xinzhi;Zhai Hong;He Shaozhen;Liu Qingchang;Su Wenjin(Food Crop Institute, He＇nan Academy of Agricultural Sciences, Zhengzhou, 450002;Key Laboratory of Sweetpotato Biology and Biotechnology of Ministry of Agriculture, China Agricultural University, Beijing, 100193;Food Crop Institute, Hubei Academy of Agricultural Sciences, Wuhan, 430064)

机构地区：[1]河南省农业科学院粮食作物研究所,郑州450002 [2]中国农业大学农业部甘薯生物学与生物技术重点实验室,北京100193 [3]湖北省农业科学院粮食作物研究所,武汉430064

出　　处：《分子植物育种》2018年第11期3569-3579,共11页Molecular Plant Breeding

基　　金：国家自然科学基金项目(U1404316);国家甘薯产业技术体系项目(CARS-10);河南省农业科学院自主创新专项基金项目(2017ZC13)共同资助

摘　　要：利用转录组数据开发SSR标记,是目前较为经济高效的DNA分子标记开发方法。为了开发更多适用于甘薯的SSR标记,本研究在前期对甘薯体细胞杂种KT1及其两个亲本4个干旱胁迫处理的24个样本转录组de novo测序的基础上,对获得的105 959条Unigene中大于等于1 000 bp的FASTA序列进行搜索,共找到12 105个SSR位点。从中筛选设计了200对SSR引物在KT1及其亲本中筛选验证,最终选择了20对多态性较好的引物,对来自不同地区的94份甘薯种质资源进行了群体聚类分析。聚类分析把94份实验材料分为3个亚群,较清晰地区分了不同材料之间的遗传相似性。这些基于甘薯转录组测序开发的SSR标记为甘薯的遗传多样性分析、辅助育种和遗传图谱构建等研究的开展提供了更加丰富的候选标记。Using transcriptome data to deve lop SSR marker is an economical and effective method of DNA molecular marker for the moment. In order to develop more SSR markers suitable for sweetpotato, we searched the FASTA sequences that were equal or longer than 1 000 bp from 105 959 Unigenes obtained based on sequencing the transcription de novo of somatic hybrid KT1 and 24 samples treated with 4 drought stresses in two parents of sweetpotato previously, and 12 105 SSR loci were found. Two hundred pairs of SSR primers were designed after fine selection, and were further verified in KT1 and its two parents. Eventually, 20 pairs of SSR primers with good polymorphism were selected for group clustering analysis of 94 sweetpotato germplasms resources from different regions. The clustering result showed that all 94 germplasms were divided into three subsets, which could clearly distinguish their genetic similarity among different materials. These SSR markers developed based on transcriptome sequencing of sweetpotato might provide more optional candidate markers for researches on sweetpotato such as genetic diversity analysis, assisted breeding, genetic map construction etc.

关 键 词：甘薯 转录组 SSR标记 聚类分析 

分 类 号：S531[农业科学—作物学]