江西铅山红芽芋低温疗法脱毒苗遗传稳定性的SSR检测  

SSR Detection of Genetic Stability of Virus-free Plantlets of Jiangxi Yanshan Red Bud Taro with Cryotherapy

作　　者：尹明华[1] 刘燕 叶思雨 宁本松 Yin Minghua;Liu Yan;Ye Siyu;Ning Bensong(College of Life Sciences, Shangrao Normal University, Shangrao, 334001)

机构地区：[1]上饶师范学院生命科学学院,上饶334001

出　　处：《分子植物育种》2018年第11期3580-3587,共8页Molecular Plant Breeding

基　　金：2016年度江西省教育厅科学技术研究项目(GJJ161053)资助

摘　　要：为了检测江西铅山红芽芋低温疗法脱毒苗是否存在遗传变异,本研究采用SSR分子标记技术与毛细管电泳技术相结合的方法来检测江西铅山红芽芋低温疗法脱毒苗的遗传稳定性。结果表明,根据PAGE检测从38对引物中选出10对引物(P10,P11,P18,P23,P24,XP3,XP5,XP7,XP8,XP10);10对引物的30个样本(包括低温疗法脱毒苗和大田苗)分型基本相同,且其多样性指数较低,观测纯合度和期望纯合度也均高于观测杂合度和期望杂合度;30个样本的遗传距离大部分均为0,少量为0.032 3、0.033 9或0.198 6,遗传相似系数大部分均为1,少量为0.968 2,表明遗传分化程度极低;按照UPGMA法进行聚类分析,30个样本聚为一类。以上研究表明,江西铅山红芽芋低温疗法脱毒苗无遗传变异,低温疗法脱毒可保证其遗传稳定性。本研究可为江西铅山红芽芋低温疗法脱毒提供遗传稳定性方面的依据。In order to detect whether there was genetic variation in the genetic stability in Jiangxi Yanshan red bud taro virus-free plantlets bywith Ccryotherapy, the method of SSR molecular markers combined with capillary electrophoresis was applied in this study to detect the genetic stability in Jiangxi Yanshan red bud taro virus-free plantlets with cryotherapy. The results showed that 10 pairs of primers（P10, P11, P18, P23, P24, XP3, XP5, XP7,XP8, and XP10） were selected from 38 pairs of primers according to the PAGE test. The typing results of 30 samples（i Including virus-free plantlets by cryotherapy and field seedlings） based on 10 pairs of primers were basically the same., Ttheir diversity index was low, and the observed heterozygosity and expected heterozygosity were also higher than the observed heterozygosity and expected heterozygosity. The genetic distances of 30 samples were mostly 0, and a few were 0.032 3, 0.033 9 or 0.198 6. The majority of genetic similarity coefficients were 1 and a small amount were 0.968 2, indicating that the degree of genetic differentiation was very low. Cluster analysis was conducted according to UPGMA method, and 30 samples were clustered into only one category. The above results showindicated that Jiangxi Yanshan red bud taro virus-free plantlets by cryotherapy had no genetic variation, and virus-free by cryotherapy could ensure its genetic stability. This study couldwhich can provide a basisreference for genetic stability of Jiangxi red bud taro virus-free by cryotherapy.

关 键 词：江西铅山红芽芋 低温疗法 脱毒苗 遗传稳定性 SSR检测 

分 类 号：S632.3[农业科学—蔬菜学]