金龙胆草组培快繁体系的建立及苦蒿素含量的测定  

Establishment of Tissue Culture and Rapid Propagation System of Conyza blinii and Determination of Blinin Content

作　　者：王涛[1] 孙蓉[1] 郑天润 孙文君 陈新 王鑫 赵佳丽 唐自钟[1] 陈惠[1] Wang Tao;Sun Rong;Zheng Tianrun;Sun Wenjun;Chen Xin;Wang Xin;Zhao Jiali;Tang Zizhong;Chen Hui(College of Life Sciences, Sichuan Agricultural University, Ya＇an, 625014)

机构地区：[1]四川农业大学生命科学学院,雅安625014

出　　处：《分子植物育种》2018年第11期3666-3674,共9页Molecular Plant Breeding

基　　金：四川农业大学211工程双支计划项目(03572096)资助

摘　　要：为建立快速高效的金龙胆草Conyza blinii H.Lév.组培快繁体系,考察不同生长时期组培样叶片中苦蒿素含量,合理利用并保护金龙胆草药材种质资源,本研究筛选野生金龙胆草幼嫩叶片为外植体,以MS和1/2 MS为基本培养基,探究组织培养各个阶段的最适培养条件。在此基础上,采用HPLC法测定不同生长时期组培样同一叶位叶片中苦蒿素的含量。结果表明,诱导愈伤组织的最适培养基配方为MS+2,4-D 1.0 mg/L+6-BA 0.1 mg/L,诱导不定芽的最佳培养基配方为MS+NAA 0.1 mg/L+6-BA 1.0 mg/L,诱导不定根的最佳培养基配方为1/2 MS+GA30.1 mg/L+IBA 0.1 mg/L。炼苗后移栽的最佳基质是腐殖质和珍珠岩2:1混合。以高效液相色谱仪对不同生长时期金龙胆草组培样叶片鲜样苦蒿素含量进行分析,结果表明移栽3个月>5个月>7个月>1个月>炼苗14 d>未炼苗。本试验建立了稳定高效的金龙胆草组培快繁体系,并成功获得再生植株,为丰富金龙胆草种资质源提供了理论依据。To establish effective and rapid tissure culture and propagation system for Conyza blinii and investigate the content of blinin in leaves of tissue culture plants at different growth stages, so as to protect and utilize Conyza blinii rationally, the tender leaves of Conyza blinii were selected as the explants in this study, and MS and 1/2 MS were taken as the minimal medium to screen out the optimum conditions for each stages of tissue culture. On the basis of this, the blinin in the same leaf position of the tissue culture plants at different growth stages were detected by HPLC. The results showed that MS＋2,4-D 1.0 mg/L＋6-BA 0.1 mg/L was the most suitable medium for callus induction; MS＋NAA 0.1 mg/L＋6-BA 1.0 mg/L was the best medium for cluster buds induction; 1/2 MS＋GA30.1 mg/L＋IBA 0.1 mg/L was the most effective medium for rooting. The best ratio of humus and perlite substrate for transplanting after seedling was 2 ： 1. HPLC results indicated that the content of blinin was as follows： transplanted for 3 monthstransplanted for 5 monthstransplanted for 7 monthstransplanted for 1 monthtrained for 14 dwithout hardening-seedling. In this experiment, a stable and efficient tissue culture and propagation system for Conyza blinii was established, and regenerated plants were obtained successfully, which could provide a theoretical basis forenriching the seed quality resources of Conyza blinii.

关 键 词：金龙胆草 愈伤组织 组培快繁 苦蒿素 HPLC 

分 类 号：R284.1[医药卫生—中药学] S567.219[医药卫生—中医学]