清脑片对小鼠脑膜微循环及血瘀模型的影响  

Effect of Qingnao Tablet on the Model of Meningeal Microcirculation and Blood Stasis in Mice

作　　者：郝少君[1] 孔学军[1] 陈维亮[1] 管志江[1] 李文俊[1] 范莉莉[1] 张正臣[1] HAO Shaojun;KONG Xuejun;CHEN Weiliang;GUAN Zhijiang;LI Wenjun;FAN Lili;ZHANG Zhengchen(The 371st Central Hospital of the Chinese People＇s Liberation Army, Xinxiang 453000, China)

机构地区：[1]中国人民解放军第三七一医院,河南新乡453000

出　　处：《中国现代应用药学》2018年第5期684-687,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：河南省科技发展计划项目(132102310408);河南新乡市重点科技创新发展专项项目(CXGG16054)

摘　　要：目的观察清脑片对小鼠脑膜微循环及血瘀模型的影响。方法60只昆明种小鼠,SPF级,♀♂各半,随机分为清脑片组、脑络通组(高、中、低剂量脑络通胶囊混悬液)、空白组及模型组6组,灌服给药,空白组及模型组灌服同体积的生理盐水,连续给药10 d,记录小鼠平静后2 min内的血流灌注量。取结扎(空白组做假手术)前110~120 s的血流灌注量平均值作为结扎前的平均灌注量,取230~240 s的血流灌注量平均值作为结扎后的平均灌注量,并进行分析。另取60只KM小鼠,SPF级,♀♂各半,随机分为6组,给药方法同前,每天给药1次,连续灌服15 d。除空白组每天后腿肌注生理盐水外,其他5组每天后腿肌注地塞米松0.8 mg·kg^(-1),连续肌注15 d。于第16天灌药及肌注后1 h,小鼠摘眼球取血,测全血黏度。结果与空白组比较,模型组脑膜微循环血流量显著减少(P<0.01)。脑络通组和清脑片高剂量组可显著改善双侧颈总动脉结扎所致的小鼠脑血流量减少(P<0.01);清脑片中剂量明显改善双侧颈总动脉结扎所致的小鼠脑血流量减少(P<0.05)。高剂量清脑片组可显著降低高切和中切黏度(P<0.01),中剂量清脑片组可明显减低高切和中切黏度(P<0.05);高、中剂量清脑片组可明显降低低切黏度(P<0.05)。结论清脑片对小鼠脑膜微循环以及小鼠血瘀模型有良好的改善作用。OBJECTIVE To observe the effect of Qingnao tablets on meningeal micro-circulation and blood stasis model of mice.METHODS Sixty Kunming mice,SPF,male and female in half,were divided into 6 groups：Qingnao tablets（large,medium,small dose）,Naoluotong capsule,the blank group and the model group.The blank and model groups were drenched normal saline of the equal volume.Then the amount of blood perfusion within 2 minutes were recorded after the mice quiet.The blood perfusion average value of 110-120 s before ligation and 230-240 s after ligation were recorded as the average perfusion value respectively.Another sixty mice,SPF,male and female half,were divided into 6 groups randomly.They were also drenched as above-mentioned method and administrated once a day for 15 d.The mice of blank group were injected with normal saline into the muscle of the back legs,while the other 5 groups were injected with an intramuscular injection of dexamethasone with a dose of 0.8 mg·kg^-1 for 15 d.The mice’s eyeballs were picked to take blood and measure the whole blood viscosity within one hour after the intramuscular injection 16 days later.RESULTS Compared with the blank group,the blood flow volume of meningeal micro-circulation in model groups was decreased significantly（P〈0.01）.The Naoluotong and Qinnao Tablets in large dose significantly improved the reduction of the mice’s brain blood flow volume caused by the legation of the bilateral carotid artery（P〈0.01）.The Qingnao tablets in medium dose significantly improved the reduction of the mice’s brain blood flow volume caused by the legation of the bilateral carotid artery（P〈0.05）.The Qingnao group in large dose significantly decreased high shear and medium shear viscosity（P〈0.01）.The Qingnao group in medium dose significantly decreasd high shear and medium shear viscosity（P〈0.05）.The Qingnao group in large and medium dose significantly decreased low shear viscosity（P〈0.05）.CONCLUSION The Qingnao tablets can well improve the meningeal mi

关 键 词：清脑片 脑膜微循环模型 血瘀模型 脑络通胶囊 

分 类 号：R285.5[医药卫生—中药学]