IgD对人外周血Th1/Th2、Th17/Treg亚群及核转录因子的影响  

Effects of IgD on the balance of Th1/Th2 and Th17/Treg subsets and transcription factors expression in human peripheral blood

作　　者：董小洁 吴育晶[1] 张静 陈文生[1] 黄琼[1] 魏伟[1] DONG Xiaojie;WU Yujing;ZHANG Jing;CHEN Wensheng;HUANG Qiong;WEI Wei(Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Collaborative Innovation Center of Anti-inflammatory and Immune Medicine in Anhui Province, Hefei 230032, Anhui, Chin)

机构地区：[1]安徽医科大学临床药理研究所抗炎免疫药物教育部重点实验室抗炎免疫药物安徽省协同创新中心

出　　处：《中国临床药理学与治疗学》2018年第5期488-497,共10页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金(81330081;81673444;81603121;81673492);安徽医科大学博士科研资助基金(XJ201629);杨森科学研究委员会中国分会研究基金(JRCC2015药理02)

摘　　要：目的:研究免疫球蛋白D(immunoglobulin D,Ig D)对人T细胞亚群Th1/Th2、Th17/Treg细胞平衡的影响。方法:不同浓度的Ig D(终浓度1、3、10μg/m L)刺激人外周血单个核细胞(peripheral blood mononuclear cells,PBMC)24 h后,流式细胞术检测CD69、CD154的表达、Th1、Th2、Th17、Treg细胞亚群的百分比,实时定量PCR(q PCR)技术检测核转录因子T-bet、GATA-3、Foxp3和ROR-γt mRNA的表达。结果:Ig D可增加T细胞表面活化标志物CD69、CD154的表达(P<0.05),显著减少Th1、Treg细胞亚群的百分比且可显著增加Th17的百分比,对Th2细胞亚群无显著影响;Ig D可显著下调核转录因子T-bet、Foxp3mRNA的表达且可显著上调核转录因子ROR-γt mRNA的表达,对核转录因子GATA-3 mRNA的表达无显著影响。结论:Ig D能促进T细胞活化,引起T细胞亚群Th1/Th2、Th17/Treg比例失衡。AIM： To investigate the effects of immunoglobulin D（ IgD） on the balance of Th1/Th2,Th17/Treg cells in healthy human T cell subsets. METHODS： Different concentrations of IgD（ final concentration of 1,3,10 μg/mL） were added to stimulate the human peripheral mononuclear cells（ PBMC）. After 24 h,the expression of CD69 and CD154 and the percentage of Th1,Th2,Th17,and Treg subsets were detected by flow cytometry. The expression of nuclear transcription factors T-bet,GATA-3,Foxp3 and ROR-γt mRNA was detected using qPCR assay. RESULTS： IgD could significantly increase the expression of CD69 and CD154（ P〈0. 05）,which are markers of activate T cells.IgD could simultaneously decrease the percentage of Th1 and Treg subsets and significantly increased the percentage of Th17 subset,but had no significant effect on Th2 subset. IgD significantly inhibited the expression of T-bet and Foxp3 mRNA and significantly promoted the expression of ROR-γt mRNA,but had no significant effect on the expression of GATA-3 mRNA. CONCLUSION： IgD can activate T cells and induce the imbalance between Th1/Th2 and Th17/Treg subsets.

关 键 词：免疫球蛋白D(IgD) T细胞亚群 核转录因子 表达 失衡 

分 类 号：R965.2[医药卫生—药理学]