地奥心血康胶囊对大鼠急性缺血心肌间隙连接蛋白43的影响  

Effects of Di ao Xin xue Kang on connexin 43 of myocardial gap junction after acute ischemia injury

作　　者：王电烨 胡志耕[1] Wang Dianye;Hu Zhigeng(Department of Cardiovascular Medicine, the Affiliated Hospital of Shanxi College of Traditional Chinese Medicine, Taiyuan, Shanxi 030024, Chin)

机构地区：[1]山西中医学院附属医院心痛科,山西省太原030024

出　　处：《中国基层医药》2018年第12期1569-1572,共4页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的观察地奥心血康胶囊对大鼠急性缺血心肌连接蛋白43（Cx43）的影响，进一步探讨其抗心律失常的可能机制。方法采用随机数字表方法将30只大鼠分成假手术组（SM组，n＝10）、缺血组（IM组，n＝10）、地奥心血康胶囊组（DA组，n＝10）。术前地奥心血康胶囊组给予胶囊粉（250 mg/kg），假手术组及缺血组均给予等量0.9%氯化钠注射液，连续干预4周。末次灌胃后，利用在体结扎冠状动脉前降支法建立急性心肌梗死模型，假手术组只穿线不结扎。观察2 h各组心律失常的发生情况；造模2 h后处死动物，应用免疫组化SP法检测Cx43在各组急性缺血心肌中的表达；应用黄嘌呤氧化酶法和硫代巴比妥酸法测各组动物血清中丙二醛（MDA）的含量和超氧化物歧化酶（SOD）的活力。结果与SM组比较，IM组心律失常评分[（3.8±0.7）分比（0.7±0.2）分，t＝13.466，P〈0.01]和血清MDA含量[（8.3±0.8）nmol/mL比（4.2±0.7）nmol/mL，t＝12.197，P〈0.01]显著增高，SOD活力[（68.2±5.5）U/mL比（144.1±7.9）U/mL，t＝24.932，P〈0.01]降低，心室肌Cx43含量[（0.39±0.06）比（0.92±0.05），t＝21.459，P〈0.01]显著降低、分布紊乱；与IM组相比，DA组心律失常评分[（2.6±0.8）分比（3.8±0.7）分，t＝3.570，P〈0.01]降低，Cx43含量[（0.60±0.08）比（0.39±0.06），t＝6.641，P〈0.01]增高、分布规律。结论地奥心血康胶囊能有效抑制大鼠急性心肌缺血心室肌Cx43降解，减少心律失常的发生，其机制可能与抗氧化有关。ObjectiveTo observe the effects of Di ao Xin xue Kang on connexin 43（Cx43）, in order to make clear the protective mechanism against ischemia-induced arrhythmias.MethodsThe rats were randomly divided into 3 groups according to the digital table： SM operation group（group SM, n=10）, ischemia group（group IM, n=10）, Di ao Xin xue Kang group（group DA, n=10）. Myocardial infarction was induced by legation the left anterior descending coronary artery for 120 min.The expression of Cx43 of the ischemic myocardium was studied by immunohistochemistry technique.The ventricular arrhythmia was observed, and MDA, SOD levels were measured.ResultsCompared with the SM group, the arrhythmia score[（3.8±0.7）points vs.（0.7±0.2）points, t=13.466, P〈0.01]and serum MDA level[（8.3±0.8）nmol/mL vs.（4.2±0.7）nmol/mL, t=12.197, P〈0.01]in the IM group were significantly increased, SOD activity in the IM group[（68.2±5.5）U/mL vs.（144.1±7.9）U/mL, t=24.932, P〈0.01]decreased, and serum Cx43 content[（0.39±0.06） vs.（0.92±0.05）, t=21.459, P〈0.01]significantly decreased.Compared with IM group, the arrhythmia score of the DA group[（2.6±0.8） vs.（3.8±0.7）, t=3.570, P〈0.01]decreased, and serum Cx43 content[（0.60 ±0.08） vs.（0.39±0.06）, t=6.641, P〈0.01]increased and distributed regularly.ConclusionDi ao Xin xue Kang can effectively alleviate Cx43 degrading induced by acute myocardial infarction, the mechanism of this effect is associated with resisting oxidative stress.

关 键 词：心肌缺血 心律失常 心性 缺血预处理 心肌 连接蛋白43 地奥心血康胶囊 

分 类 号：R965[医药卫生—药理学]