基于红外光谱技术对不同产区枸杞子的分析  

Analysis of Fructus Lycii from Different Planting Areas Based on Infrared Spectroscopy

作　　者：王秀芬[1] 李静[1] 马芳[2] 孙素琴[2] WANG Xiufen;LI Jing;MA Fang;SUN Suqin(Ningxia Polytechnic, Yinchuan 750021, China;Department of Chemistry, Tsinghua University, Beijing 100084, China)

机构地区：[1]宁夏职业技术学院,银川750021 [2]清华大学化学系,北京100084

出　　处：《医药导报》2018年第6期721-726,共6页Herald of Medicine

基　　金：宁夏高等学校项目(NGY2016252)

摘　　要：目的对不同产区枸杞子进行快速鉴别。方法采用傅里叶变换红外光谱、二阶导数谱和二维相关红外光谱技术对不同产区枸杞子的药材粉末及其水提物和醇提物进行分析与评价。结果不同产区枸杞子的一维光谱非常相似,相似系数均>0.950;二阶导数谱中不同产区枸杞子原药材和水提物在1 500~1 800 cm-1的峰位和峰强差异明显。在800~1 160 cm-1波段内二维相关谱中,宁夏枸杞子的相对峰强度最高,反映宁夏枸杞子的多糖类及糖苷类物质均高于其他3个产区;在1 520~1 800 cm-1波段内,宁夏枸杞子有2组明显自动吸收峰,内蒙古、甘肃、青海枸杞子有4组明显自动吸收峰,更直观反映出不同产区枸杞子的差异。不同产区水提物和醇提物的分析结果进一步明确不同产区枸杞子中氨基酸、蛋白质、酰胺类、多糖及糖苷类物质存在明显差异。结论红外光谱法结合二维相关红外光谱技术为不同产区枸杞子的鉴别和分析提供一种快速、全面的评价方法。Objective To rapidly identify Fructus Lycii in different planting areas. Methods Fourier transform infrared spectroscopy（FTIR） and two-dimensional correlation spectroscopy（2 DIR） were used to analyze and evaluate the medicinal powder,water extract and alcohol extract of Fructus Lycii in different planting areas. Results The result of singledimensional FTIR of Fructus Lycii in different planting areas were very similar,and the similarity coefficients were more than 0.950. In the two order derivative spectrum,the peak position and the peak intensity of crude medicinal herbs and water extracts of Fructus Lycii were obviously different at 1 500-1 800 cm-1. At the 800-1 160 cm-1 band in the two-dimensional correlation spectrum,the relative peak intensity of Ningxia wolfberry was the highest. It indicated that the polysaccharides and glycosides of Fructus Lycii in Ningxia were higher than those in other three areas. In the 1 520-1 800 cm-1 band,there were 2 groups of obvious automatic absorption peaks in Ningxia wolfberry. There were 4 groups of obvious automatic absorption peaks in Inner Mongolia,Gansu and Qinghai wolfberry,which reflected the difference of Fructus Lycii in different area. Through analysis of water extracts and alcohol extracts of Fructus Lycii from different planting areas,it further indicated that there were obvious differences in the amino acids,proteins,amides,polysaccharides and glycosides in Fructus Lycii from different planting area.Conclusion FTIR with 2 DIR provided a rapid and comprehensive evaluation method for identification and analysis of Fructus Lycii in different planting areas.

关 键 词：枸杞子 傅里叶变换红外光谱 二维相关红外光谱 

分 类 号：R282.1[医药卫生—中药学]