慢性乙醇中毒对雌雄小鼠海马mTOR通路相关蛋白影响  

Effect of chronic alcohol poisoning on mTOR pathway related proteins in hippocampus of mice

作　　者：邢阳[1] 邹丹[1] 廖海清 张雅婷 于桉国 唐小雨 吕鑫 李晓成 XING Yang;ZOU Dan;LIAO Hai-qing(Department of Pathophysiology, Shenyang Medical College, Shenyang, Liaoning Province 110034, China)

机构地区：[1]沈阳医学院病理生理教研室 [2]沈阳医学院

出　　处：《中国公共卫生》2018年第6期834-838,共5页Chinese Journal of Public Health

基　　金：辽宁省自然科学基金(No.201602735);国家级大学生科研创新项目(No.201610164010);辽宁省大学生科研创新项目(No.201610164010);沈阳医学院大学生科研创新项目(No.20160803)

摘　　要：目的探讨慢性乙醇中毒对雌雄小鼠认知影响的差异,明确m TOR信号通路相关蛋白的变化,揭示慢性乙醇中毒引起脑损伤的机制。方法将120只8周龄昆明种雌雄小鼠随机分为对照组(蒸馏水灌胃)、12.5%、25%、50%乙醇给药组(0.1 m L/10 g),给药9周。Morris水迷宫检测小鼠学习记忆能力,Western blot法检测各组小鼠p-m TOR及p-Akt蛋白表达。结果 Morris水迷宫定位航行结果表明:雌鼠及雄鼠对照及低中高剂量乙醇实验组第5 d逃避潜伏期分别为[(24.49±8.20),(28.34±9.88),(29.13±12.55),(34.46±13.13),(24.46±5.05),(36.02±9.17),(38.76±6.63),(43.06±8.06)s]较第1 d[(54.64±5.23),(55.22±4.62),(56.25±3.43),(58.33±0.85),(54.16±3.91),(55.69±4.16),(56.89±2.80),(58.34±1.44)s]明显缩短(P<0.05)。空间探索结果表明:50%乙醇组雌鼠及25%、50%乙醇组雄鼠有效区停留距离分别为[(20.88±2.73),(20.19±3.07),(10.33±3.07)cm]较其对照组[雌(37.72±1.78)cm;雄(37.65±6.31)cm]明显缩短(P<0.05)。50%乙醇组雄鼠有效区停留距离[(10.33±3.07)cm]较同浓度组雌鼠[(20.88±2.73)cm]明显缩短(P<0.05)。Western blot结果表明:50%乙醇组雄鼠海马p-Akt(2.75±0.42)、p-m TOR(0.59±0.10)蛋白水平较对照组(1.20±0.51,0.34±0.14)明显升高(P<0.05),但雌鼠却没有明显变化。结论慢性乙醇中毒对学习记忆的影响存在性别差异,m TOR信号通路可能参与此过程。Objective To explore different effects of chronic alcohol poisoning on cognition function of male and female mice and to reveal the change in mammalian target of rapamycin （mTOR） signaling pathway related proteins and the mechanism of brain injury induced by chronic alcoholpoisoning. Methods Totally 120 Kunming mice of 8-week old （half male and half female） were randomly assigned into low-, moderate-, and high-dose experimental groups with intragastric administration of 12.5%, 25%, and 50% ethanol at the dosage of 0.1 ml/10 g.day and a control group administrated with equal volume of distilled water. The administrations were applied once a day consecutively for 9 weeks. Then, learning and memory ability of the mice were determined with Morris water maze; relative expressions of phosphorylated mTOR （p-mTOR） and phosphorylated protein kinase B （p-Akt） in hippocampus of the mice were detected with Western blot. Results Compared to those determined at the first day of Morris water maze test, the escape latency time （seconds） at the fifth day was significantly shortened for the mice of the control, low-, moderate-, and high-dose ethanol groups （for female mice： 24.49 ± 8.20 vs. 54.64 ± 5.23, 28.34 ± 9.88 vs. 55.22 ± 4.62, 29.13 ± 12.55 vs. 56.25 ± 3.43, and 34.46 ± 13.13 vs. 58.33 ± 0.85; for male mice： 24.46 ± 5.05 vs. 54.16 ± 3.91, 36.02 ± 9.17 vs. 55.69 ± 4.16, 38.76 ± 6.63 vs. 56.89 i 2.80, 43.06 ± 8.06 vs. 58.34 ± 1.44, respectively） （P 〈 0.05 for all）. In spatial probe trials, the female mice in high-ethanol group and the male mice in moderate-, and high-ethanol groups showed significantly lower travel distance （centimeters） in effective zone compared to the mice of control group （for the female mice： 20.88 ± 2.73 vs. 37.72 ± 1.78; for the male mice 20.19 ± 3.07 and 10.33 ± 3.07 vs. 37.65 ± 6.31） （P 〈 0.05 for all）; for the mice of high-ethanol group, the males showed significantly lower travel distance in effective zone than the females （10

关 键 词：乙醇 海马 学习记忆 MTOR信号通路 

分 类 号：R338.2[医药卫生—人体生理学]