富H2水对电离辐射所致认知功能损伤的保护作用  

The protective effect of hydrogen-rich water on irradiation.induced cognitive dysfunction

作　　者：刘梦雅[1] 宋建波 高洁 袁慧[1] 王永丽[1] 王新钢[1] 岳娟[1] 秦秀军[1] 李建国[1] 闻建华[1] Liu Mengya;Song Jianbo;Gao Jie;Yuan Hui;Wang Yongli;Wang Xingang;Yue Juan;Qin Xiujun;Li Jianguo;Wen Jianhua(Key Laboratory of Drug Toxicology and Drug for Radiation Injury of Shanxi Province, Institute for Radiation Protection, Taiyuan 030006,Chin;Department of Radiotherapy, Shanxi Dayi Hospital, Taiyuan 030032, China)

机构地区：[1]中国辐射防护研究院药物毒理与放射损伤药物山西省重点实验室,太原030006 [2]山西大医院放疗科,太原030032

出　　处：《中华放射医学与防护杂志》2018年第6期414-418,465,共6页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的 探讨富H2水对电离辐射所致大鼠认知功能损伤的保护作用。方法 将20只SD大鼠利用随机数表法分为对照组（C）、富H2水组（HRW）、单纯照射组（IR）和富H2水干预组（HRW＋IR）4个组，每组5只。通过Morris水迷宫对各组大鼠空间记忆能力进行检测；对超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、丙二醛（MDA）、8-羟基脱氧鸟苷（8-OHdG）等氧化应激指标的变化进行检测；通过实时荧光定量PCR反应对凋亡相关基因表达情况进行研究。结果 大鼠逃避潜伏期4组间差异有统计学意义（F=6.003，P〈0.05），4组间的平台象限游泳距离差异具有统计学意义（F=3.850，P〈0.05）。其中，与IR组相比，HRW＋IR组第3、4和5天的逃避潜伏期明显缩短（P〈0.05），原平台象限游泳距离显著延长（P〈0.05）。4组间的GSH、8-OHdG、MDA差异均具有统计学意义（F=6.450、5.033、4.113，P〈0.05），与IR组相比，脑组织中GSH浓度显著升高（P〈0.05），而MDA和8-OHdG浓度显著降低（P〈0.05）。PCR结果显示，与IR组相比，HRW＋IR组caspase-3、caspase-9基因表达水平均显著下降（t=2.956、3.087，P〈0.05），bcl-2基因表达水平显著升高（t=-3.640，P〈0.05），bax基因表达水平显著降低（t=5.246，P〈0.05）。结论 富H2水可能通过中和氧羟自由基，减少电离辐射的氧化损伤作用，从而影响细胞凋亡过程进而对大脑产生保护作用。Objective To investigate the protective effect of hydrogen-rich water on rat cognitive dysfunction induced by ionizing radiation. Methods A total of 20 SD rats were randomly divided into four groups with the ramdom number table method：control group （C）, hydrogen-rich water group （HRW）, irradiation group （IR） and hydrogen-rich water intervention group （HRW＋IR）, with 5 rats in each group. The spatial memory ability of rats was tested by a morris water maze. The expression of apoptosis-related genes was detected by real-time fluorescence quantitative PCR. The changes of glutathione （GSH）, 8-hydroxydeoxy guanosine （8-OHdG） and malondialdehyde （MDA） and SOD were also measured. Results The escape latency （F=6.003, P〈0.05） and the swimming distances （F=3.850, P〈0.05） of rats in four different groups had statistically significant differences. Compared with the IR group, the escape latency of the HRW＋IR group was significantly decreased at 3, 4, 5 d after irradiation （P〈0.05）, and the swimming distance of this group became much longer （P〈0.05）. The levels of GSH, 8-OHdG and MDA in these four groups had statistically significant differences （F=6.450, 5.033, 4.113, P〈0.05）. Compared with IR group, the concentration of GSH was increased （P〈0.05）, but MDA and 8-OHdG decreased （P〈0.05） in the brain tissue of HRW＋IR group, and the expressions of caspase-3, caspase-9 and bax genes were reduced （t=2.956,3.087, 5.246, P〈0.05）, while the expression of bcl-2 gene was enhanced （t=-3.640, P〈0.05） in the HRW＋IR group. Conclusions Hydrogen-rich water attenuates the oxidative damage of ionizing radiation by neutralizing oxyhydrogen free radicals and thus protects brain from radiation damage.

关 键 词：富H2水 电离辐射 认知功能损伤 辐射防护 

分 类 号：R818[医药卫生—放射医学]