基于UPLC-LTQ Orbitrap HRMS分析天芪降糖胶囊的化学成分  

Identifications of components of Tianqi Jiangtang Capsules by UPLC-LTQ Orbitrap HRMS

作　　者：何晨婕 彭丰 刘彤[1] 彭静波[1] HE Chen-jie;PENG Feng;LIU Tong;PENG Jing-bo(Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008, China)

机构地区：[1]中南大学湘雅医院临床药理研究所,湖南长沙410008

出　　处：《中草药》2018年第9期2019-2025,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目:基于HPLC-TPMT EAD-MS联用技术的天芪降糖胶囊中TPMT酶亲和活性成分研究(81403086)

摘　　要：目的运用超高液相色谱-双压线性离子阱高分辨率组合型质谱联用(UPLC-LTQ Orbitrap HRMS)技术对天芪降糖胶囊化学成分进行分析和鉴定。方法采用Waters ACQUITY UPLC?BEH C18色谱柱(100 mm×2.1 mm,1.7μm),流动相(p H≈3.5)为甲酸-甲酸铵缓冲盐溶液(A)-甲醇(B),梯度洗脱,ESI采用正负离子扫描模式。根据精确相对分子质量、二级质谱裂解碎片和色谱峰保留时间等信息结合对照品的碎片裂解规律,以及对照品对照和文献数据进行结构鉴定。结果通过文献比对、裂解规律及对照品对比共鉴定出17个化合物,其中包括9个生物碱、3个糖苷、3个有机酸、2个黄酮类化合物。结论该研究为天芪降糖胶囊的活性研究、质量控制及临床应用提供了化学基础。Objective To analysis and identify the components of Tianqi Jiangtang Tables. Methods The components of Tianqi Jiangtang Capsules were separated on Waters ACQUITY UPLC BEH C18 column（100 mm × 2.1 mm, 1.7 μm）. The mobile phase consisted of ammonium formate（A） and methol（B） using a gradient elution（buffer p H ≈ 3.5）. The mass spectrometer was operated in both positive and negative mode, and the main chromatographic peaks were detected by UPLC-LTQ/Orbitrap/MS/MS. Compounds were identified on the basis of their accurate molecular weight and MSn fragment data, or by comparing the relative retention time and the mass spectrometric data of the standard substance and consulting the reference literature. Results A total of 17 compounds were identified, including 9 alkaloids, 3 glycosides, 3 organic acids, and 2 flavonoids. Conclusion The results of components identification can provide the evidence for pharmacological studies, quality control, and clinical application of Tianqi Jiangtang Capsules.

关 键 词：天芪降糖胶囊 超高液相色谱-双压线性离子阱高分辨率组合型质谱联用 生物碱 糖苷 黄酮 

分 类 号：R284.1[医药卫生—中药学]