栀子全时段融合指纹图谱的构建及特征峰的鉴定  

Study on all-time multi-wavelength fusion fingerprints of chemical components in Gardeniae Fructus and quantitative analysis of characteristic components

作　　者：王伟[1,2] 付娟[1,2] 万琴[1,2] 李家春[1,2] 黄文哲[1,2] 王振中[1,2] 萧伟[1,2] WANG Wei;FU Juan;WAN Qin;LI Jia-chun;HUANG Wen-zhe;WANG Zhen-zhong;XIAO Wei(Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China)

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中草药》2018年第9期2158-2162,共5页Chinese Traditional and Herbal Drugs

基　　金：重大新药创制项目科技重大专项"现代中药创新集群与数字制药技术平台"(2013ZX09402203)

摘　　要：目的建立栀子的指纹图谱并采用电喷雾离子源-四极杆-飞行时间质谱(ESI-Q-TOF MS)对其共有峰进行定性分析。方法采用Kromasil 100-5 C18柱,以乙腈-0.1%磷酸梯度洗脱,体积流量为1 m L/min,柱温为35℃,检测波长为238、327、440 nm。使用Matlab 7.1软件编程,对3波长下的CSV格式数据进行全时段融合。结果各色谱峰分离度良好,3波长融合指纹图谱全面体现了栀子238、327、440 nm特征吸收波长的指纹信息,标定20个共有峰,并完成其中16个成分的鉴定。10批不同批次的栀子指纹图谱与对照指纹图谱之间相似度较好,相似度大于0.90以上。结论实现了栀子融合指纹图谱的测定,同时解析了图谱中的化学组成,并制定了主成分与栀子苷相对峰面积的范围,为全面控制药材质量提供了数据支撑。Objective To establish the multi-wavelength fusion HPLC fingerprints of Gardeniae Fructus and to make quantitative analysis of the common peaks by ESI-Q-TOF MS. Methods The Kromasil 100-5 C18 column was used with a mobile phase of acetonitrile-0.1% phosphoric acid in gradient elution; The flow rate was 1.0 m L/min; The column temperature was 35 ℃; The detection wavelength was 238, 327, and 440 nm. Matlab7.1 was adopted for multi-wavelength fusion of the data in CSV format. Results Twenty chemical constituents were detected in fusion fingerprint, which included more information. Sixteen chemical constituents were compared with reference standards and identified by MS. The similarity of 10 batches of Gardeniae Fructus was over 0.90. Conclusion The systematic quantified fingerprint method based on the technique of multi-wavelength fusion fingerprint and identifying 16 constituents can be used for the fully quality control of Gardeniae Fructus.

关 键 词：栀子 全时段融合指纹图谱 山栀苷 京尼平苷酸 去乙酰车叶草酸甲酯 京尼平龙胆双糖苷 栀子苷 新绿原酸 绿原酸 隐绿原酸 异绿原酸A 西红花苷-Ⅰ 西红花苷-Ⅱ 

分 类 号：R286.6[医药卫生—中药学]