青藤碱对淋巴瘤Daudi细胞增殖及α7nAChR表达的影响  

Effects of Sinomenine on Proliferation of Lymphoma Daudi Cells and Expression of Alpha 7nAChR

作　　者：石清平 易浪 白莎莎 植颖坤 王培训 董燕 SHI Qing-Ping;YI Lang;BAI Sha-Sha;ZHI Ying-Kun;WANG Pei-Xun;DONG Yah(Dept. of Immunology , lnstitute of Clinical Pharmacology , Guangzhou University of Chinese Medicine, Guangzhou510405 Guangdong, China)

机构地区：[1]广州中医药大学临床药理研究所免疫研究室,广东广州510405

出　　处：《广州中医药大学学报》2018年第4期693-698,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81573672);广东省自然科学基金资助项目(编号:2017A030310502)

摘　　要：【目的】探讨青藤碱(SIN)对Burkkit淋巴瘤Daudi细胞增殖及α7烟碱型乙酰胆碱受体(α7nAChR)表达的影响。【方法】采用CCK-8法观察Daudi细胞的生长特点。分别以青藤碱及对照药物烟碱(Nic)、甲氨喋呤(MTX)干预48 h后,采用CCK-8法检测各组Daudi细胞增殖情况,采用流式细胞术检测各组细胞凋亡率,采用蛋白免疫印迹(Western blot)法检测Daudi细胞α7nAChR蛋白表达,采用逆转录聚合酶链反应(RT-PCR)法检测α7nAChR mRNA表达。【结果】与空白对照组比较,青藤碱抑制Daudi细胞增殖,增加细胞早期凋亡率;Nic促进Daudi细胞增殖,降低凋亡率;MTX抑制Daudi细胞增殖,增加凋亡率。Western blot和RT-PCR检测发现青藤碱降低Daudi细胞α7nAChR蛋白和mRNA表达,Nic增加Daudi细胞α7nAChR蛋白和mRNA表达,MTX对Daudi细胞中α7nAChR蛋白和mRNA表达没有明显影响。【结论】青藤碱对Burkkit淋巴瘤Daudi细胞增殖具有抑制作用并抑制α7nAChR表达,与MTX抗淋巴瘤细胞增殖的机制不同。Objective To investigate the effects of sinomenine（SIN）on the proliferation of Burkkit lymphoma Daudi cells and the expression of alpha 7 nicotinic acetylcholine receptor（α7nAChR）. Methods CCK-8 method was used to observe the growth of Daudi cells. After treatment with SIN and control medicines of nicotine（Nic）,methotrexate（MTX）respectively for 48 h,the effect on Daudi cell proliferation was detected by CCK-8 assay,and the apoptotic rate,the expression levels of α7nAChR protein and mRNA were detected by flow cytometry,Western blotting method, reverse transcription-polymerase chain reaction（RT-PCR）, respectively. Results Compared with the blank control group,SIN inhibited the proliferation and increased the early apoptotic rate of Daudi cells;Nic promoted the proliferation and decreased the apoptotic rate;MTX inhibited the proliferation and increased the apoptotic rate. Western blotting and RT-PCR assay results showed that SIN reduced the expression levels of α7nAChR protein and mRNA,Nic increased the expression levels of α7nAChR protein and mRNA,and MTX had no effect on the expression levels of 7 n ACh R protein and mRNA in Daudi cells. Conclusion SIN can inhibit the proliferation of Burkkit lymphoma Daudi cells and inhibit the expression of α7nAChR, and its therapeutic mechanism is different from that of MTX in counteracting the proliferation of lymphoma cells.

关 键 词：青藤碱 烟碱 甲氨喋呤 淋巴瘤细胞 细胞增殖 α7烟碱型乙酰胆碱受体(α7nAChR) 

分 类 号：R285.5[医药卫生—中药学]