电针对缺氧缺血性脑病幼鼠学习记忆能力的影响  

Effect of Electroacupuncture on Learning-memory Ability in Rats with Hypoxic-ischemic Encephalopathy

作　　者：潘友灿 许能贵[1] 易玮[1] 史俊恒[1] 郑晓燕[1] PAN You can;XU Neng-gui;YI Wei;SHI Jun-heng;ZHENG Xiao-yan(Southern China Research Center for Acupuncture and Moa＇ibustion , Guangzhou University of Traditional Chinese Medicine ,Guangzhou 510006 , China)

机构地区：[1]广州中医药大学华南针灸研究中心,广州510006

出　　处：《针刺研究》2018年第6期370-374,共5页Acupuncture Research

基　　金：国家自然科学基金重点项目(No.81230088)

摘　　要：目的:观察电针对缺氧缺血性脑病幼鼠学习记忆能力的影响,探讨电针对其神经元的作用机制。方法:将7d龄SD大鼠随机分为假手术组(n=12)、模型组(n=11)和电针组(n=12)。采用左颈总动脉结扎联合缺氧的方法建立缺氧缺血性脑病模型。电针组予电针"百会""大椎",隔日1次,每次20min,连续28d。观察治疗后幼鼠转棒跌落潜伏期、高架迷宫进入开放臂时间、水迷宫逃避潜伏期和逃避距离,高尔基染色法观察幼鼠神经元树突棘的密度。结果:转棒跌落潜伏期和高架迷宫进入开放臂时间,3组之间差异无统计学意义(P>0.05)。水迷宫实验,模型组较假手术组逃避潜伏期延长(P<0.05),逃避距离增加(P<0.05),平台象限停留时间减少(P<0.05),原平台穿越次数减少(P<0.05);电针组较模型组逃避潜伏期缩短(P<0.05),逃避距离减少(P<0.05),平台象限停留时间增加(P<0.05),原平台穿越次数增加(P<0.05)。模型组较假手术组树突棘密度降低(P<0.05),电针组较模型组树突棘密度升高(P<0.05)。结论:电针可以改善幼鼠缺氧缺血造成的学习记忆能力损伤,其作用可能与调节神经元树突棘密度有关。Objective To observe the effect of electroacupuncture （EA） on changes of learning-memory ability, psychomotor coordination and anxiety-like behavior of cerebral hypoxic-ischemia （CHi） young rats, so as to explore its protective effect on neurons under hypoxic-ischemic conditions. Methods SD rats （aged 7 days） were randomly divided into sham operation （sham, n = 12）, model （n = 11）, and EA groups （n = 12）. In addition, 6 young rats in each group were used for observing the number of dendritic spines after Golgi staining. The CHI model was established by ligation of the left common carotid artery combined with hypoxia in a closed transparent vessel. EA was applied to ＂Baihui＇（GV 20）and ＂Dazhui＂ （GV 14） for 20 min, once every other day, for 28 days. The rats＇ behavior changes were assessed by using rotarod performance （for psychomotor coordi- nation）, elevated plus maze （anxiety-like behavior） tests and Morris water maze （learning-memory ability） tests, separately. Results After modeling, the average escape latency and average escape distance of location navigation test within 70 seconds were significantly increased （ P〈0.05）, and the average times and average duration of safe-platform quadrant crossing of spacial probing test were markedly reduced relevant to the sham group （P〈0.05）. After EA treatment, CHI-induced increases of escape latency and escape distance, and the decreased times and duration of platform quadrant crossing were significantly reversed （P〈0.05）. No significant differences were found among the three groups in the falling latency of rotarod performance test, and in the time of opening and closing arms of elevated plus maze tests （ P〉0.05）. The density of dendritic spines was significantly lo- wer in the model group than in the sham group （P 〈0.05）, and notably higher in the EA group than in the model group （P〈0.05）. Conclusion EA can improve the learning-memory ability of OHI young rats, which may be related

关 键 词：电针 脑缺氧缺血 学习记忆能力 海马CA 1区 神经元 树突棘 

分 类 号：R245.9[医药卫生—针灸推拿学]