磷霉素氨丁三醇散对慢性细菌性前列腺炎模型大鼠前列腺组织TNF-α、IL-8、IL-6含量的影响  

Fosfomycin tromethamine inhibits the expressions of TNF-α,IL-8 and IL-6 in the prostate tissue of rats with chronic bacterial prostatitis

作　　者：蔡文伟[1] 莫敦胜 范明 蔡鸿财 张国巍[5] 王卫萍 商学军[5] CAI Wen-wei;MO Dun-sheng;FAN Ming;CAI Hong-cai;ZHANG Guo-wei;WANG Wei-piong;SHANG Xue-jun(Center of Reproductive Medicine, Jiaxing Women and Children＇s Hospital, Jiaxing, Zhejiang 314000, China;Department of Urology, Liuzhou Workers＇Hospital / The Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi 545005, China;Institute of Medical Laboratory;Family Planning Research Institute / Center of Reproductive Medicine, Hua- zhong University of Science and Technology, Wuhan, Hubei 430030, China;Department of Andrology, Jinling Hospital Affiliated to Southern Medical University / Nanjing General Hospital of Nanjing Military Region, Nanjing, Jiangsu 210002, China)

机构地区：[1]嘉兴市妇幼保健院生殖中心,浙江嘉兴314000 [2]广西医科大学第四附属医院/柳州市工人医院泌尿外科,广西柳州545005 [3]华中科技大学计划生育研究所/生殖医学中心,湖北武汉430030 [4]南方医科大学医学院附属金陵医院/南京总医院临床中心实验科,江苏南京210002 [5]南方医科大学医学院附属金陵医院/南京总医院男科,江苏南京210002

出　　处：《中华男科学杂志》2018年第6期491-498,共8页National Journal of Andrology

基　　金：2017年度军队计生专项研究任务计划(17JS013);江苏省社会发展面上项目(BE2017724)~~

摘　　要：目的:探讨磷霉素氨丁三醇散(FT)对慢性细菌性前列腺炎模型大鼠肿瘤坏死因子-α(TNF-α)、白介素8(IL-8)、白介素6(IL-6)表达水平的影响。方法:70只雄性SD大鼠随机分为7组,每组10只。A组:假手术组;B组:模型对照组;C组:阳性对照组[左氧氟沙星:100 mg/(kg·d),30 d];D组:FT低剂量、14 d疗程组[200 mg/(kg·d),14 d];E组:FT低剂量、7 d疗程组[200 mg/(kg·d),7 d];F组:FT高剂量、14 d疗程组[300 mg/(kg·d),14 d];G组:FT高剂量、7 d疗程组[300 mg/(kg·d),7 d],各组均采用灌胃给药。实验结束后留取各组大鼠前列腺组织,制作病理切片并使用酶联免疫吸附实验(ELISA)检测各组大鼠前列腺组织匀浆中TNF-α、IL-8、IL-6的含量。结果:与空白对照组比较,模型组大鼠前列腺组织匀浆中的TNF-α、IL-8、IL-6含量均明显升高,差异均有统计学意义[(19.83±6.1)ng/g prot vs(32.93±6.21)ng/gprot,(8.26±0.52)ng/g prot vs(16.2±2.84)ng/g prot,(1.55±0.11)ng/g prot vs(2.51±1.06)ng/g prot,P<0.05或0.01];与模型组[(32.93±6.21)ng/g prot、(16.2±2.84)ng/g prot]相比,各治疗组大鼠前列腺组织匀浆中的TNF-α、IL-8含量[(20.54±5.78)ng/g prot、(21.95±6.48)ng/g prot、(23.8±6.93)ng/g prot、(19.97±2.58)ng/g prot、(21.97±3.38)ng/g prot;(12.43±3.64)ng/g prot、(11.11±2.86)ng/g prot、(12.43±4.02)ng/g prot、(8.83±1.32)ng/g prot、(12.68±1.97)ng/g prot]明显降低,差异具有统计学意义(P<0.05或0.01);各治疗组大鼠前列腺组织匀浆的TNF-α、IL-8、IL-6含量与阳性对照组比较差异均无统计学意义(P>0.05);F组大鼠前列腺组织匀浆中的IL-6的表达量较模型组显著减少[(2.51±1.06)ng/g prot vs(1.76±0.46)ng/g prot,P<0.05],其余治疗组及阳性对照组前列腺组织匀浆中的IL-6的表达与模型组比较均无显著性差异(P>0.05),但较模型组均有下降趋势。结论:FT通过抑制TNF-α、IL-8、IL-6的表达,减轻前列腺组织的炎症反应,达到治疗大鼠CBP的目的,为临床研究提供了实验�Objective： To investigate the effects of fosfomycin tromethamine （FT） on the expressions of tumor necrosis factor- α （TNF-α）, interleukin-8 （IL-8）, and interleukin-6 （IL-6） in the prostate tissue of the rats with chronic bacterial prostatitis （CBP）. Methods： We randomly divided 70 male SD rats into 7 groups of equal number： blank control, CBP model control, positive control, 14 d low-dose FT, 7 d low-dose vr, 14 d high-dose FT, and 7 d high-dose FT. The CBP model rats in the latter five groups were trea- ted intragastrically with levofloxacin at 100 mg/kg/d for 30 days and FT at 200 mg/kg/d for 14 and 7 days and at 300 mg/kg/d for 14 and 7 days, respectively. Then we collected the prostate tissue from the animals for determination of the levels of TNF-α, IL-8 and IL- 6 by ELISA. Results： Compared with the blank controls, the CBP model rats showed significantly increased levels of TNF-α （[19.83 ± 6.1] vs [32.93 ± 6.21] ng/g prot, P 〈0.01）, IL-8 （[8.26 ± 0.52] vs [16.2 ± 2.84] ng/g prot, P 〈0.01） and IL-6 （ [1.55 ±0. 11] vs [2.51 ± 1.06] ng/g prot, P 〈0.05） in the prostate tissue. In comparison with the CBP model con- trois, the levels of TNF-α and IL-8 were remarkably decreased in the groups of positive control （ [ 20.54 ± 5.78 ] ng/g prot, P 〈 0.01; [12.43± 4.02] ng/g plot, P 〈0.05）, 14 d low-dose FT （[21.95 ± 6.48] ng/g prot, P 〈0.01; [11. 11 ±2.86] ng/gprot, P 〈0.01）,7 dlow-doseFT （[23.8 ± 6.93] ng/gprot, P 〈0.05; [12.43 ± 4.02] ng/gprot, P 〈0.05）, 14 d high-dose FT （ [ 19.97 ± 2.58 ] ng/g prot, P 〈 0.01 ; [ 8.83 ＋ 1.32 ] ng/g prot, P 〈 0.01 ）, and 7 d high-dose FT （ [ 21.97 ±3.38] ng/g prot, P 〈0.01 ; [ 12.68 ± 1.97] ng/g prot, P 〈0.05）. No statistically significant differences were observed between the positive control and FY groups in the contents of TNF-α, IL-8 or IL-6 （P 〉 0.05）. The expression of IL-6 was markedly reduced in the 14 d high-dose FT group as compared with the model controls （

关 键 词：磷霉素氨丁三醇散 慢性细菌性前列腺炎 肿瘤坏死因子-α 白介素8 白介素6 大鼠 

分 类 号：R697.33[医药卫生—泌尿科学]